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Short-term toxicity to aquatic invertebrates

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Description of key information

In the static immobilization test, young Daphnia magna STRAUSS (< 24 h old) were exposed to the test item for 48 hours according to OECD 202 (2004). The EC50 value was determined to be nominal 9.29 mg test item/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
9.29 mg/L

Additional information

The acute toxicity of the test substance to Daphnia magna (STRAUS) was determined in a study (BASF SE, 2016) according to OECD 202 (2004). The effects of the test substance with a nominal loading of 0 (control), 0.46, 1.0, 2.2, 4.6 and 10 mg/L were determined under GLP conditions. The corresponding mean measured concentrations of the test substance were determined to be 0.441, 0.958; 2.134, 4.469, 9.825 mg/L. The study encompassed 4 replicates per concentration and 1 replicate per control. The number of the organisms per vessel was 5. The mobility of the Daphnia was determined in a static test by visual observation after 24 and 48 hours. The stock solution was prepared by directly adding of the test substance to the test medium and stirring for about one day. No undissolved test substance was observed. The lower test concentrations were prepared by dilution of this stock solution. The samples collected at the start of the exposure (0 h) from additionally prepared test vessels without daphnids and at the end of the exposure (48 h) from vessels with daphnids (pooled replicates) were analyzed via HPLC. A reference substance sodium chloride was used as positive control. The EC50 of the reference substance after 48 hours was determined to be 4.37 g/L. This result was within the range of 3.88 – 7.22 g/L and indicates that the culture of Daphnia magna used in this study was responding normally to toxic stress. The water pH, temperature and dissolved oxygen were within acceptable guideline specifications. The toxicity results presented here were consistent with the results from preliminary tests. Immobilization (mortality) and sublethal effects were observed daily. The analytically determined concentrations of the test substance in the test solutions were within ± 20 % of the nominal concentrations, therefore the effect concentrations can be expressed relative to the nominal concentration for the evaluation of the test substance. The EC50 value was determined to be 9.29 mg test item/L. All reported results refer to the nominal concentrations.