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EC number: - | CAS number: -
Disintegration per minute (dpm) and stimulation index for the 4 test group and the vehicle control:
dpm/node dpm/8 as 8 lymph nodes were pooled per group.
The skin sensitization of the test item was investigated in a local lymph node assya in CBA/Ca strain female mice according to OECG Tg 429 and EU method B42 and under GLP. The concentrations applied were determined in a prelimianry study and no signs of toxicity or irritation were observed at a concentration of 50% w/w of the test article in dimethyl formamide (DMF). In the main study groups of 4 mice were applied 25 micro-l of a 0 (vehicle control), 10, 25 or 50% suspension of the test article on the dorsal parts of both ears for 2 consecutive days. At day 5 3H-Thymidine was injected into the tail vein of each animal (20 micro-Ci/mouse). The stimulationm index was determined from the preparation of single cell suspensions of the auricular lymph nodes of each mouse pooled per dose group by liquid scintillation counting. The stimulation index was expressed as the mean radioactive incorporation for each treatment group divided by the mean radioactive incorporation of the vehicle control group. The criterium for a positve result was a 3 fold increase compared to controls. The test item did not show a positive response in this assay. The stimulation idices were 0.89 (10% test item in DMF), 1.48 (25% test item in DMF) and 0.9 (50% test item in DMF)
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