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EC number: - | CAS number: -
Temperatures measured throughout the period of sludge maintenance and during the test ranged from 21.5 – 22.3°C. The initial total suspended solids (TSS) concentration of the activated sludge after removal of the supernatant layer measured 13,780 mg/L. The measured TSS concentration of the sludge on the day of testing was 3813 mg/L. The measured pH of the sludge on the day of testing was 7.8.
The respiration rates observed in the two controls were 86.4 and 86.4 mg O2/L/hr, or a difference of 0.0%. The EC50 value for the reference substance was 11.8 mg/L and was within the 5 to 30 mg/L range considered acceptable for the test. Confidence limits (95%) for the EC50 and the EC50 value itself, were calculated using binomial probability with nonlinear interpolation. The observed percent inhibitions for AMP (Amino Methyl Propanol) ranged from –29.6 to 85.9%
The effect of AMP (Amino Methyl Propanol) on activated sludge microorganisms maintained in an
aerobic environment was assessed by the Activated Sludge Respiration Inhibition Test Method (OECD
Guideline 209). The test contained control, reference and treatment groups. The control replicates were
used to determine the background respiration rate of the sludge and were not dosed with the test or
reference substance. The reference group was dosed with 3,5-dichlorophenol, a known inhibitor of
respiration, at concentrations of 3, 15 and 50 mg/L. The treatment group was dosed with AMP (Amino
Methyl Propanol) at concentrations of 10, 30, 100, 300 and 1000 mg/L. After an exposure period of three
hours, the respiration rates of the test solutions were measured using a YSI Model 50B-115 Dissolved
Oxygen Meter. The respiration rates in the two controls were 86.4 and 86.4 mg O2/L/hr. The difference
between the two control respiration rates was 0.0 %, and was within the 15% difference limit established
for the test. The validity of the test was further supported by the results from the 3,5-dichlorophenol
reference group, which resulted in an EC50 value of 11.8 mg/L, with 95 percent confidence limits of
3 mg/L and 15 mg/L. The EC50 for the reference substance was within the 5 to 30 mg/L range considered
acceptable for the test. An inhibitory dose response effect was observed for the treatment group. The
EC50 value for AMP (Amino Methyl Propanol) was determined to be 342.9 mg/L. The following is a
summary of the results:
Nominal Concentrations mg/L
Respiration Rate mg O2/L/hr1
Percent Respiration Inhibition1
AMP (Amino Methyl Propanol)
1Calculations performed using MS Excel 2000 in full precision mode. Manual calculations may differ slightly.
NA - Not Applicable
Test Concentration - Control #1, Control #2, Positive Control, and 100, 180, 320, 580, 1000 mg/L
Respiratory Rate (mg O2/L/h) - 29.2, 33.6, 16.8, 28.8, 28.4, 28.4, 27.6, 23.2
Inhibition (%) - 0, 0, 47, 8, 10, 10, 12, 26
Both the studies on AMP and PTSA show that no effects are expected at 100 mg/L. Therefore the NOEC is set at 100 mg/L
The rationale for read-across can be found in the document attached in section 13.
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