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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Irritation / corrosion
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not applicable
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: meets generally accepted scientific standards, well-documented, and acceptable for assessment
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: Methods of Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians" (USEPA, 1975
- Deviations:
- no
- Remarks:
- Not specified in report
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Deionized water was used in the preparation of all stock solutions.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnids (Daphnia magna) first instar < 24 hours old. The daphnia used in the test were cultured at the testing facilities, and the adult daphnia were fed a suspension of trout chow and alfalfa daily until 24 hours prior to testing.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 225 ppm as CaCO3
- Test temperature:
- 19-20C
- pH:
- 8.1 in the control, 8.3 (100mg/L AMP), 8.9 (320 mg/L AMP), and 9.4 (1000 mg/L AMP)
- Dissolved oxygen:
- Dissolved oxygen levels ranging from 8.3 mg/L in the control at study start, and levels of 8.5 (control), 8.5 (100mg/L AMP), 8.6 (320 mg/L AMP), and 8.6 mg/L O2 (1000 mg/L AMP) at 48 hours.
- Nominal and measured concentrations:
- Nominal- 0, 100, 180, 320, 560, and 1000 mg/L AMP-95
- Details on test conditions:
- Daphnias (Daphnia magna) first instar < 24 hours old. Test conditions were a temperature range of 19-20C, and a photoperiod of 16 hours light/ 8 hour dark photcycle. Test solution (200mL) was placed in 250mL beakers, and ten daphnias were added per test solution.
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 193 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 100-320 mg/L
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 240 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 212-272 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- There was no additional data provided on the results of the range-finding study referenced in the methods section.
- Reported statistics and error estimates:
- A computerized program calculated the LC50 for Daphnia based on the Probit dose-response model.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 48-hour Daphnia magna LC50 was calculated to be 193 mg/L.
- Executive summary:
AMP-95 (P-2031, 2-amino-2-methyl-l-propanol), lot number 216 (AMP-94.31%, Water 5.69%, NVM 0.0004%, Neutral Eq. 94.68), was tested for the acute t toxicity effects on daphnias. The 24 and 48 hr LC50 and "no effect" levels were determined by ABC Laboratories, Inc., Columbia, Missouri. Test Conditions-daphnias (Daphnia magna) first instar ≤24 hr old. Test temperature 19-20C. Photoperiodicity 16 hr day light. 200 ml test solution in 250 ml beakers. Ten daphnias per test solution. The AMP-95 concentrations were tested in duplicate at 0, 100, 180, 320, 560 and 1000 mg/liter (ppm). Results- There was no significant changes in the water quality parameters except an increase in pH at the ≥320 mg/l concentration. The estimated LC50 at 24 hr was 240 (212-272) mg/liter and at 48 hr was 193 (100-320) mg/liter (95 percent confidence limits are in parenthesis). The 48 hr “no effect” level was 100 mg/liter.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: A well documented study, but the exposure duration is shorter than the guideline requires
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- exposure duration is 24h and not 48h
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 5000, 2000, 1000, 100, 10 mg/L
- Sampling method: confirmation of stock solution but not of test concentrations
- Sample storage conditions before analysis: no data - Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: potassium dichromate - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: daphnia
- Strain: Straus
- Source: in laboratory breed
- Age at study initiation: >3h, <24h
- Method of breeding: adult animals were cultivated in 4L glass vessels at 21C. The medium was changed once per week. The medium was synthetic water according to DIN 38 412 Part 11. Fed twice daily with algae (Scenedesmus supspicatus).
- Feeding during test: no
ACCLIMATION
- Acclimation period: in house culture and culture medium and test dilution medium are the same - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Hardness:
- synthetic water according to DIN 38 412 Part 11
- Test temperature:
- 21C
- pH:
- synthetic water according to DIN 38 412 Part 11
- Dissolved oxygen:
- not reported
- Nominal and measured concentrations:
- Nominal concentrations - 1000, 100, 10 mg/L and control in a study conducted on March 7, 1983; and 5000 and 2000 mg/L and a control in a study conducted on March 22, 1983.
No measured concentrations - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 100 mL
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per positive control (replicates): 2
- Biomass loading rate: no data
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water according to DIN 38 412 Part 11
- Culture medium different from test medium: no, they are the same
- Intervals of water quality measurement: no data
OTHER TEST CONDITIONS
- Light intensity: dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility at 24 hours - Reference substance (positive control):
- yes
- Remarks:
- 1.5 mg/L potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- 10% immobilization in blank control in 3 concentration test conducted on March 7, 1983
15% immobilization at 10 mg/L
5% immobilization at 100 mg/L
35% immobilization at 1000 mg/L
0% immobilization in blank control in 2 concentration test conducted on March 22, 1983
0% immobilization at 2000 mg/L
10% immobilization at 5000 mg/L - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality: 100% at 1.5 mg/L in both tests conducted on March 7 and on March 22, 1983
- EC50/LC50: expected to be in the range of 0.6 and 2.1 mg/L; suggesting that the daphnids used in this study were at least as sensitive and perhaps more sensitive than anticipated for this species. - Validity criteria fulfilled:
- yes
- Remarks:
- except exposure duration less than the required 48h
- Conclusions:
- This well-documented, but short-duration study provides weight of evidence that toluene-4-sulphonic acid has a relatively low toxicity to daphnids, with an EC50 > 1000 mg/L (equivalent to >650 mg/L active substance).
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not applicable
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: meets generally accepted scientific standards, well-documented, and acceptable for assessment
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1045 (Penaeid Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- The test species is a brown shrimp from the North Sea. No analytical verification of the test concentrations was made.
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):Not applicable
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Each test solution was prepared by introducing the appropriate weight of test material into 10 litres of clean seawater in the exposure vessel.
- Test organisms (species):
- Crangon crangon
- Details on test organisms:
- TEST ORGANISM- Common name: Brown shrimp- Source: Tor Bay- Weight at study initiation (mean and range, SD): average weight 1.9g- Length at study initiation (length definition, mean, range and SD): average length 49.8 mmACCLIMATION- Acclimation period: 5 days on site fed with a specific diet and a minimum of 3 days at the test temperature.
- Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- No data
- Test temperature:
- 14.7-15.8C
- pH:
- 8.0-10.1
- Dissolved oxygen:
- 7.2-8.4 mg/L
- Salinity:
- 34.86-34.90 o/oo
- Nominal and measured concentrations:
- Nominal- 0, 56, 100, 320, and 560 mg/L
- Details on test conditions:
- Concentrations of AMP-95 tested were 0, 56, 100, 320, and 560 mg/L under semi-static conditions for 96 hours. The 18L glass vessel was folled with 10L of the test solution, and 20 brown shrimp were added to each vessel after an on-site acclimation period of 5 days on a specially-prepared diet. Food was withheld during the test. The test solution was comprised of local filtered seawater (pH=8.10-8.16, salinity 34.86-34.90 o/oo), and was maintained within a temperature range of 14.7-15.8C. The solutions were aerated with compressed air and dissolved O2 ranged from 7.2-8.4 mg/L. The pH range during the test was 8.0-10.1. Hardness was not reported.
- Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 241 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI: 17.8-292 mg/L
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 179 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 98.2-329 mg/L
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 179 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 98.2-329 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 179 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 98.2-329 mg/L
- Details on results:
- The control mortality was 0% at 96 hours.
- Results with reference substance (positive control):
- Not applicable
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The Crangon crangon 96 hour LC50 was 179 mg/L.
- Executive summary:
A sample of a biocide, 2 Amino-2-Methyl-Propanol, (AMP 95), (Brixham Test Substance No K349), was submitted by CEGB for
aquatic toxicity testing. The species tested were the brown shrimp (Crangon crangon) and the plaice (Pleuronectes platessa). Each test solution was prepared by introducing the appropriate weight of the test material into 10 litres of clean seawater in the exposure vessel. The test organisms were than added to the seawater. Certificates of the test results are given and the cumulative mortalities in each concentration have been tabulated. The shrimp test was carried out during the period 15-19 August 1983 and the plaice test during the period 22-26 August 1983. The COD, TOC, and BOD of the product were also measured by standard methods and are reported below. The study numbers were K349/A and K349/B. All records and original data relevant to these studies are stored in the Brixham archive. Conclusion: Cumulative percentage mortality.
Referenceopen allclose all
Not applicable
Description of key information
Short term toxicity studies on invertebrates on AMP show EC50 values of 193 mg/L in fresh water and 179 mg/L in salt water. The 24 h EC50 for daphnia in a test with PTSA is > 650 mg/L.
For the substance the EC50 is set at 193 mg/L in a worst case assumption. No value for salt water is derived in absence of data on PTSA.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 193 mg/L
Additional information
The rationale for read-across can be found in the document attached in section 13.
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