Reaction product of alkylarylsulphonic acid and cycloaliphatic amine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not applicable

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: meets generally accepted scientific standards, well-documented, and acceptable for assessment

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

other: Methods of Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians. Stephan, C. E., Chairman. 1975. Committee on Methods for Toxicity Tests with Aquatic Organisms. U.S. EPA, Ecol. Res. Ser. 660/3-75009.

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):Not applicable

Analytical monitoring:

no

Details on sampling:

- Concentrations:0, 32, 56, 100, 180, 320 and 560 mg/l, with ten fish per concentration. Before the test concentrations were prepared, the standard was allowed to warm to room temperature (22°C). The test concentrations were obtained by transferring appropriate aliquots of a working solution directly to the test chambers. The working solution was prepared in deionized water,

Vehicle:

no

Details on test solutions:

Prepared in deionized water. Test concentrations were prepared based on the total compound.

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

Bluegill sunfish were obtained from a commercial supplier, and held in culture tanks with a 16-hour light cycle, and acclimated for 14 days prior to exposure to the test material. Fish received a standard commercial fish food until 48 hours prior to testing, at which time feeding was discontinued. The fish had a mean weight of 0.21 grams and a mean standard length of 22 mm.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Hardness:

45 mg/L as CaCO3total alkalinity was 25 mg/L CaCO3

Test temperature:

21-23C

pH:

7.0

Dissolved oxygen:

8.8 mg/L

Nominal and measured concentrations:

Dose levels for the definitive assay were 32-560 mg/L in logarithmic series. Concentrations are reported based on nominal dose.Dose levels for the definitive 96-hour study were set in a 72-hour range-finding test at 1, 10, 100, 560, 1000 mg/L.

Details on test conditions:

The static bioassay was conducted in 5-gallon glass vessels containing 15 liters of reconstituted water (48mg NaHCO3, 30mg CaSO4, 30mg MgSO4, and 2mg KCl). Vessels were kept in a water bath at 21-23C, and the fish were acclimated to the dilution water and test temperature, and fasted for 48 hours prior to testing.

Reference substance (positive control):

yes

Remarks:

Antimycin A

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

190 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 150-250 mg/L

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mortality and sublethal effects

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 170-280 mg/L

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 170-280 mg/L

Details on results:

The LC50 data was reported as follows:AMP 24-hour LC50 = 220 (170-280) mg/LAMP 48-hour LC50 = 220 (170-280) mg/LAMP 96-hour LC50 = 190 (150-250) mg/LLoss of equilibrium and surfacing was noted in some fish at 320 mg/L.

Results with reference substance (positive control):

The LC50 data was reported as follows:Antimycin A 24-hour LC50 = 0.000056 mg/LAntimycin A 48-hour LC50 = 0.000037 mg/LAntimycin A 96-hour LC50 = 0.000028 mg/L

Sublethal observations / clinical signs:

The LC50 was determined with the Probit method

Validity criteria fulfilled:

not specified

Remarks:

There is no information on the stability of the test subtance. No mortality was observed in the control.

Conclusions:

The 96h LC50 and NOEC were 190 mg/L and 100 mg/L, respectively.

Executive summary:

The acute toxicity of AMP-95 to bluegill sunfish (Lepomis macrochirus) was assessed using the methods outlined by the Committee on Methods for Toxicity Tests with Aquatic Organisms. Water quality parameters of temperature, dissolved oxygen, pH and ammonia were measured throughout the test and were within acceptable limits. As a quality check, the bluegill sunfish were challenged with a reference compound, Antimycin A. The observed 96 hour LC50 and 95% confidence limits (C.I.) were within the 95% confidence limits reported in the literature, indicating that the fish were in good condition. The results of the four day static fish toxicity study using bluegill sunfish are: 96h-LC50 = 190 (150 -250 mg/L).

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Method similar to guideline but no analytical, and test species is not the standard species

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

no chemical analysis; Goldorfen is not the standard test species but is among those often tested

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

diluted in water only

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: Goldorfen
- Source: Paul Eggers, 2345 Hohenwestedt
- Age at study initiation (mean and range, SD): not indicated
- Length at study initiation (length definition, mean, range and SD): 5.5 - 6.6 cm
- Weight at study initiation (mean and range, SD): 1.5-2.7 g
- Feeding during test: no

ACCLIMATION
- Acclimation period: no indicted
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min
- Feeding frequency: not indicated
- Health during acclimation (any mortality observed): not indicated

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

114 mg CaCO3/L

Test temperature:

20 +/- 1 C

pH:

7.5 - 8.2 (except at 500 mg/L where it was 5.7)

Dissolved oxygen:

8.5 - 9.2 mg/L

Salinity:

N/A

Nominal and measured concentrations:

Nominal - 0, 10, 100 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium with 20L solution
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 20L
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not indicated

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Ca/mg ratio: 0.7
- Na/K ratio: 21
- Intervals of water quality measurement: 0, 2, 24, 48, 72 and 96 hours

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12
- Light intensity: 700 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality daily; observations regularly

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 5-10X
- Range finding study: not indicated

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

>325 mg/L based on active ingredient

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no difference compared to control group
- Observations on body length and weight: not indicated
- Other biological observations: macroscopic examination showed no changes
- Mortality of control: none

Validity criteria fulfilled:

yes

Conclusions:

Test substance was not toxic at 500 mg/L (325 mg/L active substance), the highest concentration tested.

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

Not applicable

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: meets generally accepted scientific standards, well-documented, and acceptable for assessment

Reason / purpose for cross-reference:

reference to same study

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

Deviations:

yes

Remarks:

A saltwater species was tested. No analytical verification of the test concentrations

Principles of method if other than guideline:

The test protocols are appended to the report.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

Concentrations were prepared in sea-water.

Test organisms (species):

Pleuronectes platessa

Details on test organisms:

- Length at study initiation (length definition, mean, range and SD): mean length of 63.2mm- Weight at study initiation (mean and range, SD): mean weight of 4.1g - Nb of fish per vessel : 20 - Source: Tor Bay

Test type:

semi-static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

not reported

Test temperature:

Nominal temperature of the test solution was 15C (14.4-15C during the test).

pH:

8.1-10.3

Dissolved oxygen:

7.0-8.2 mg/L

Salinity:

34.76-34.82 o/oo

Nominal and measured concentrations:

Nominal concentrations tested were 100, 320, 560, 1000 mg/L.

Details on test conditions:

In a 96-hour, semi-static seawater test system, the toxicity of AMP-95 to Plaice was studied. Fish (20 per dose level, mean weight of 4.1g and mean length of 63.2mm) were placed in 18L glass vessels containing 10L of the test solution. The pH of the dilution seawater was 8.08-8.14, and salinity was 34.76-34.82 o/oo. Concentrations tested were 100, 320, 560, 1000 mg/L.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

231 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 138-385 mg/L

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

184 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 31-340 mg/L

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

184 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 31-340 mg/L

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

184 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 31-340 mg/L

Reported statistics and error estimates:

The LC50 were determined based on the Probit model.

Sublethal observations / clinical signs:

Not applicable

Validity criteria fulfilled:

yes

Remarks:

No mortality was observed in the control, the DO ranged between 7.2-8.4 mg/L and the substance is not hydrolysable.

Conclusions:

The Pleuronectes platessa 96 hour LC50 was 184 mg/h (95 CI:31-340 mg/l).

Executive summary:

A sample of a biocide, 2 Amino-2-Methyl-Propanol, (AMP 95), (Brixham Test Substance No K349), was submitted by CEGB for aquatic toxicity testing. The species tested were the brown shrimp (Crangon crangon) and the plaice (Pleuronectes platessa). Each test solution was prepared by introducing the appropriate weight of the test material into 10 litres of clean seawater in the exposure vessel. The test organisms were than added to the seawater. Certificates of the test results are given and the cumulative mortalities in each concentration have been tabulated. The COD, TOC, and BOD of the product were also measured by standard methods and are reported below. Conclusion: Cumulative percentage mortality

Description of key information

Short term toxicity studies on fish on AMP show LC50 values of 190 mg/L in fresh water and 183 mg/L in salt water. The LC50 for fresh water fish in a test with PTSA is > 325 mg/L.

For the substance the LC50 is set at 190 mg/L in a worst case assumption. No value for salt water is derived in absence of data on PTSA.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

190 mg/L

Additional information

The rationale for read-across can be found in the document attached in section 13.