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Biological Oxygen Demand (BOD): The time required for biodegradation to exceed 10% DO2 (i.e., the lag period) was 7.9 days and the 60% DO2 level was exceeded after 9.6 days. By the end of the 28- day test, biodegradation of the test substance reached 89.3 ± 10.8% DO2 (mean ± 1SD).
CO2 Evolution: Biodegradation of the substance exceeded 10% DCO2 after 6.9 days and after 28 days reached 90.9 ± 2.9% DCO2 (mean ± 1SD). Therefore, the rates and extents of biodegradation determined from CO2 evolution closely reflected those determined from the BOD values.
DOC Analyses DOC analyses also indicated that biodegradation of the test substance occurred under the conditions of this test. The initial DOC concentration (mean ± 1 SD) in the Test Suspensions was 5.86 ± 0.1 mg/L, which indicated that the test substance components were completely dissolved in the inoculated mineral medium. The DOC concentration after 28 days was 0.110 ± 0.1 mg/L, which equates to a 98.1% reduction of the initial DOC concentration.
The complete biodegradation of aniline indicated from measurements of BOD and CO2 evolution was confirmed by analyses of DOC removal. DOC was removed by 98.6% in both replicate Positive Control mixtures.
The recorded values from the temperature reference vessel averaged 21.9 ± 0.4°C (± 1SD, n=17), and the minimum and maximum recorded temperatures were 21.0 and 22.8°C, respectively, over the entire duration of this test.
The pH of the biodegradation reaction mixtures remained within the required range of 7.2 to 7.6 over the duration of this test. The pH of the Test Suspensions decreased by no more than 0.29 pH units from their initial values over 28 days, and showed only a 0.06 pH unit (maximum) difference relative to the Inoculum Blanks at the end of the test. This minimal variation in pH indicates that the mineral medium contained adequate buffering capacity for the inoculum and test substances evaluated in this test.
The Toxicity Control mixtures containing 100 mg/L aniline and 10.9 mg/L of the test substance showed no evidence for inhibition of the microbial inoculum by the test substance. The onset and rate of biodegradation in the Toxicity Control mixture closely matched that in the Positive Controls further indicating that the test substance did not cause toxicity to or inhibition of the microbial inoculum.
A single Abiotic Control mixture was included in the experimental design to determine the extent to which abiotic processes may result in degradation of the test substance. The mixture contained 11.0 mg/L of test substance in the inoculated mineral medium, which was chemically sterilized by addition of 250 mg/L HgCl2. The Abiotic Control mixture exhibited no O2 consumption or CO2 production over the duration of the 28-day test (data not shown). Therefore, the O2 consumption and CO2 production in the Test Suspensions was solely attributed to biodegradation of the test substance.
The ready biodegradability of AMP (2-amino-2-methyl-1-propanol) was evaluated using the OECD Guideline No. 301F: Manometric Respirometry Test. The test employed biodegradation reaction mixtures (500 mL) containing a defined mineral medium, which was inoculated with 30 mg/L activated sludge (dry solids) collected from the City of Midland Wastewater Treatment Plant (Midland, Michigan). Biodegradation of AMP was evaluated at a concentration of 11.1 mg/L, which was equivalent to 21.8 mg/L theoretical oxygen demand (ThOD). The onset of AMP biodegradation (i.e. BOD ≥ 10% of theoretical oxygen demand, ThOD) occurred after 7.9 days in the Test Suspensions, and biodegradation exceeded the pass level of 60% ThOD consumption within 1.7 days thereafter. At the end of the 28-day test, the extent of biodegradation based on BOD, CO2 evolution, and dissolved organic carbon (DOC) removal reached 89.3 ± 10.8%, 90.9± 2.9%, and 98.1 ± 1.7% (mean ± 1 Std. Dev.), respectively. Thus, biodegradation exceeded both the pass level and 10-day window criteria for classification as “readily biodegradable”. The Toxicity Control mixture showed no apparent toxic or inhibitory effects of the AMP substance on the inoculum employed in this test; and no net consumption of oxygen or DOC removal was observed in the Abiotic Control mixture. All test parameters met the OECD-specified validation criteria for ready biodegradability tests. Biodegradation of the reference compound aniline exceeded the pass level within 6.6 days, and thus confirmed the viability of the inoculum. Other experimental parameters, such as reaction mixture pH, incubation temperature, and total oxygen consumption in the Inoculum Blanks, fell within the ranges required by the OECD guideline. Therefore, the results of this test indicate that AMP does meet OECD criteria for “ready biodegradability” in the Manometric Respirometry test.
PTSA was tested in a CO2 evolution test and showed >99% biodegrdation in 28 days.The extent of biodegradation of AMP based on BOD, CO2 evolution, and dissolved organic carbon (DOC) removal reached 89.3 ± 10.8%, 90.9± 2.9%, and 98.1 ± 1.7% (mean ± 1 Std. Dev.).
Based on the information on the components the substance is expected to be readily biodegradable.
The rationale for read-across can be found in the document attached in section 13.
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