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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
(Q)SAR
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
accepted calculation method
Remarks:
Internationally accepted method, ECOSAR, EPA (USA)
Justification for type of information:
See attached (Q)SAR prediction reporting format (QPRF).
Principles of method if other than guideline:
QSAR calculation by ECOSAR v1.1, EPA, USA (attached QSAR Prediction Reporting Format, QPRF)
GLP compliance:
no
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
364.06 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
not applicable
Conclusions:
The estimated 48-h LC50 for Daphnia is 364.060 mg/l (ECOSAR v1.1, neutral organics).
Executive summary:

The estimated 48 -h LC50 for Daphnia is 364.060 mg/l (ECOSAR v1.1, neutral organics).

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Read-across from an analogue substance for which a guideline study is available (Klimish score=4 because secondary source)
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH
See attached reporting format.
Reason / purpose:
read-across source
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
634.41 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Based on a read-across from an analogue from which EC50 = 980 mg/L
Validity criteria fulfilled:
yes
Conclusions:
Based on the read-across approach from the analogue 1,1,1,2-tetrafluoroethane, the 48h-EC50 of 1,1-difluoroethane was determined to be 634.41 mg/l based on mobility.
Executive summary:

An acute toxicity test with the analogue substance 1,1,1,2 -tetrafluoroethane on Daphnia magna was performed in accordance with the EU Method C.2 (Acute Toxicity for Daphnia). The test duration was 48 hours. The acute test with Daphnia magna showed a steep concentration-immobility curve. At mean measured concentrations of 870 and 1,100 mg/l the immobility after 48 h was 0 and 100%, respectively. The 48h-EC50 was determined to be 980 mg/l based on mobility. On the basis of these results, the read-across approach was applied and the 48h-EC50 based on mobility of 1,1 -difluoroethane was determined to be 634.41 mg/L.

Description of key information

Key study: Internationally accepted method, ECOSAR, EPA (USA). The estimated 48-h EC50 for aquatic invertebrates is 364.060 mg/l.

Supporting study: Secondary source. Test method according to EU Method C.2. GLP study. Based on the read-across approach from the analogue 1,1,1,2-tetrafluoroethane, the 48h-EC50 of 1,1-difluoroethane was determined to be 634.41 mg/l based on mobility.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
364.06 mg/L
EC50/LC50 for marine water invertebrates:
364.06 mg/L

Additional information

Key study: The estimated 48-h EC50 for aquatic invertebrates is 364.060 mg/l (ECOSAR v1.1, EPA, USA).

Supporting study: An acute toxicity test with the analogue substance 1,1,1,2 -tetrafluoroethane on Daphnia magna was performed in accordance with the EU Method C.2 (Acute Toxicity for Daphnia).The test duration was 48 hours.The acute test with Daphnia magna showed a steep concentration-immobility curve. At mean measured concentrations of 870 and 1,100 mg/l the immobility after 48 h was 0 and 100%, respectively. The 48h-EC50 was determined to be 980 mg/l based on mobility. On the basis of these results, the read-across approach was applied and the 48h-EC50 based on mobility of 1,1 -difluoroethane was determined to be 634.41 mg/l.