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Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From April 27, 2016 to November 08, 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, adapted
Details on inoculum:
- Source of inoculum/activated sludge: Dihua sewage treatment plant, Taiwan.
Duration of test (contact time):
29 d
Initial conc.:
1 g/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance:
acetic acid, sodium salt
Remarks:
Sodium acetate
Parameter:
% degradation (CO2 evolution)
Remarks on result:
other: The percentage biodegradation of Evercion SR61 was not be exceed 10 % and percentage biodegradation was not achieve 60 % with following 10 days.
Results with reference substance:
At Day 14 during the study, the biodegradation was 69.33 %.

At the end of the test, the CO2 production of blank control groups were 38.09 mg/L.

 

Table 1. The percentage biodegradation of the test groups

Day

Percentage of biodegradation (% ThCO2)a

Test group

Flask 1

Flask 2

Mean

Difference of replicateb

2

0.86

1.43

1.14

-0.57

5

1.14

1.14

1.14

0.00

7

1.90

2.47

2.19

-0.57

9

3.42

2.09

2.76

1.33

14

4.18

2.28

3.23

1.90

19

5.61

2.38

3.99

3.23

23

6.75

2.76

4.75

3.99

27

7.99

2.85

5.42

5.13

29

9.41

3.33

6.37

6.09

a Percentage of biodegradation (% ThCO2) = (cumulative CO2of test group (mg) -cumulative CO2of blank control group (mg)) / (added TOC (mg) × 3.67) ×100%

b Difference of replicate = Flask 1- Flash 2

 

Table 2. The percentage biodegradation of the reference and toxicity control group

Day

Percentage of biodegradation (% ThCO2)a

Referencecontrol group

Toxicity control group

Flask 5

Flask 6

2

21.44

11.31

5

47.79

23.33

7

60.59

29.66

9

66.77

31.67

14

69.33

32.48

19

71.15

33.43

23

73.49

33.63

27

75.09

34.39

29

75.20

34.54

a Percentage of biodegradation (% ThCO2) = (cumulative CO2of reference control group or toxicity control (mg) - cumulative CO2of toxicity control group (mg)) / (added TOC (mg) × 3.67) ×100%

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
According to OECD 301B test method, Evercion SR61 was not be considered readily biodegradable.
Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100099001EN which is based on the SOP for the OECD 301B (SOPE-503) and OECD 301B (OECD, 1992). The results of this OECD 301B test for Evercion SR61 show that all validity criteria were met. Evercion SR61 was not readily biodegradable under the test conditions and the percentage biodegradation of Evercion SR61 in sludge was less than 60 %.

Description of key information

Not readily biodegradable (OECD TG 301B).

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable, not fulfilling specific criteria

Additional information

The total amount of evolved CO2 in the blank control group was 38.09 mg/L. The difference between replicates of test group was less than 20%. At Day 14 during the study, the biodegradation of the reference control article was 69.33 % and the percent biodegradation in the toxicity control was 32.48 %. All test validity criteria were met. Evercion SR61 was not readily biodegradable under the test conditions and the percentage biodegradation of Evercion SR61 in sludge was less than 60 %.