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EC number: 944-086-6 | CAS number: -
Title: The test item: Toxicity to the Aquatic Plant Lemna gibba in a Semi-Static Growth Inhibition Test
Commission Regulation (EC) No 761/2009, Annex, Part C, C.26.: "Lemna sp. Growth Inhibition Test", Official Journal of the European Union (EN), dated August 24, 2009
OECD Guideline 221: "Lemna sp. Growth Inhibition Test", adopted March 23, 2006
SANCO/3029/99 rev.4 11/07/00: Residues: Guidance for generating and reporting methods of analysis in support of pre-registration data requirements for Annex II (part A; Section 4) and Annex III (part A; Section 5) of directive 91/414
OECD Series on Testing and Assessment, No. 23, "Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures", December 15, 2000
The influence of the test item on the growth of the freshwater plant Lemna gibba was assessed in a semi-static concentration-response test.
The 7-day EyC50 was calculated to be > 100 mg test item/L for frond number and dry weight, respectively.
The 7-day ErC50 was calculated to be > 100 mg test item/L for frond number and dry weight, respectively.
The 7-day NOEyC and the LOEyC were determined to be 32 and 100 mg test item/L for frond number and dry weight, respectively.
The 7-day NOErC and the LOErC were determined to be 32 and 100 mg test item/L for frond number and dry weight, respectively.
The quantification of the test item in the test samples was performed using liquid chromatography with UV detection. In the freshly prepared test media at the start of the test and at the renewal of the test media 96% of the nominal test concentrations were found (average of all test concentrations). In the aged test media at day 2, day 5 and day 7 of the test duration, 90% of the nominal value was determined (average of all test concentrations). During the test the test organism were exposed to a mean of 93% of nominal. Therefore, all reported results refer to nominal concentrations.
This study is classified acceptable and satisfies the guideline requirements for aquatic plant studies.
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