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EC number: 944-086-6 | CAS number: -
Percentage Biodegradation of Test Item and of the Toxicity Control based on ThODNO3and of Sodium Benzoate based on ThODNH4
Flask 1 [%]
Flask 2 [%]
Flask 5 [%]
Flask 7 [%]
1COD of Duasyn-Tintenblau SLK: 1.117 mg O2/mg test item 2ThODNH4of sodium benzoate: 1.666 mg O2/mg reference item
pH-Values at the End of the Test
Reference item (procedure control)
Test item: Ready Biodegradability in a Manometric Respirometry Test
This study is designed to comply with the following methods:
- Commission Regulation 440/2008/EC, Method C.4-D of May 30, 2008: Manometric Respirometry Test (EEC Publication No. L 142/496, May 2008)
- OECD Guideline for Testing of Chemicals No. 301 F: "Ready Biodegradability: Manometric Respirometry Test", adopted July 17, 1992
Material and Methods:
Aerobic activated sludge (microorganisms from a domestic wastewater treatment plant) was supplied by the sewage treatment plant of Rossdorf, Germany.
The test item was investigated for its ready biodegradability in a manometric respirometry test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
Degradation rate of test item calculated by the oxygen consumption of the aerobic activated sludge microorganisms after 28 days of incubation.
Test Item Loading Rate (initial concentration in medium C0):
103.7 mg/L corresponding to an oxygen demand of about 1.117 mg O2/mg TS (COD)
Reference Item Loading Rate:
102.9 mg/L corresponding to an oxygen demand of about 171.4 mg/L (ThODNH4)
22°C ± 1°C, darkness
Biodegradation of the test item:
The test item contains nitrogen; therefore the evaluation of biodegradation has to be based ThODNH4and ThODNO3. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the 10-day window, describing the period between reaching at least 10% degradation and 60% degradation. This period should not exceed 10 days.
The mean biodegradation of 10% of the test item was never reached. The mean biodegradation at test end after 28 days was 7% (ThODNO3).
Therefore, the test item is considered to be not readily biodegradable based on ThODNO3.
Biodegradation of Sodium Benzoate:
The reference item sodium benzoate was sufficiently degraded to 76% after 14 days and to 85% after 28 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
Biodegradation of the Toxicity Control:
In the toxicity control containing both, the test item and the reference item sodium benzoate, 43% (ThODNO3) biodegradation was noted within 14 days and 48% (ThODNO3) biodegradation after 28 days of incubation. According to the test guidelines, the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days.
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