Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically acceptable method

Data source

Reference
Reference Type:
publication
Title:
Comparative Effects of Heterocyclic Compounds on Inhibition of Lettuce Fruit Germination
Author:
Reynolds T
Year:
1989
Bibliographic source:
Journal of Experimental Botany 40 (212): 391-404.

Materials and methods

Principles of method if other than guideline:
- Fruit germination inhibition test according to Reynolds (1975)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Phthalide
EC Number:
201-744-0
EC Name:
Phthalide
Cas Number:
87-41-2
Molecular formula:
C8H6O2
IUPAC Name:
1(3H)-Isobenzofuranone
Details on test material:
- Name of test material (as cited in study report): Phthalide
- Analytical purity: not reported

Sampling and analysis

Analytical monitoring:
not specified

Test substrate

Vehicle:
not specified

Test organisms

Species:
Lactuca sativa
Plant group:
Dicotyledonae (dicots)

Study design

Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
other: agar (0.5%)
Total exposure duration:
3 d

Test conditions

Test temperature:
30 °C
Details on test conditions:
TEST SYSTEM
- Testing facility:
- Test container (type, material, size): Plastic containers sealed with snap-on lids
- Number of replicates: 5

Results and discussion

Effect concentrationsopen allclose all
Species:
Lactuca sativa
Duration:
3 d
Dose descriptor:
EC50
Effect conc.:
14.8 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
germination
Species:
Lactuca sativa
Duration:
3 d
Dose descriptor:
EC50
Effect conc.:
22.2 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
germination

Any other information on results incl. tables

In addition the EC50 -value was converted to mg/kg soil dw. Since the amount of agar in the test was with 0.5% negligiable, the EC50 of 14.8 mg/L was related to mg/kg water and multiplicated with the factor of 1.5 (standard soil density) to get mg/kg soil dw.

Applicant's summary and conclusion