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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study (with GLP)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Phthalide
EC Number:
201-744-0
EC Name:
Phthalide
Cas Number:
87-41-2
Molecular formula:
C8H6O2
IUPAC Name:
1(3H)-Isobenzofuranone
Details on test material:
- Batch number: 7375191-5
- Molecular formular: C8H6O2
- Molecular weight [g/mol]: 134
- Aggregate state: solid
- Colour: yellow-white
- Purity of the test substance: 99.0%
- Impurities: no information
- Homogeneity: yes (melting and mixing)



Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from laboratory wastewater treatment plant fed with municipal and synthetic sewage
- Pretreatment: none

Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
ca. 28 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- pH: 7.38 - 7.41

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Test performed in open system: yes

SAMPLING
- Sampling frequency: every 2-4 days for at least 28 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Procedure control: yes
- Abiotic sterile control: yes
- Adsorption control: yes
- Toxicity control: yes
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
> 90 - < 100
Sampling time:
28 d
Details on results:
> 50% after 5 days
>=95% after 7, 10, 14, 17, 21, 24, 27, 28 days

BOD5 / COD results

Results with reference substance:
are valid (>=90% after 5, 7, 10, 14, 17, 21, 24, 27, 28 days)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable