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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study (with GLP)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Phthalide
EC Number:
201-744-0
EC Name:
Phthalide
Cas Number:
87-41-2
Molecular formula:
C8H6O2
IUPAC Name:
1(3H)-Isobenzofuranone
Details on test material:
- Batch number: 7375191-5
- Molecular formular: C8H6O2
- Molecular weight [g/mol]: 134
- Aggregate state: solid
- Colour: yellow-white
- Purity of the test substance: 99.0%
- Impurities: no information
- Homogeneity: yes (melting and mixing)



Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from laboratory wastewater treatment plant fed with municipal and synthetic sewage
- Pretreatment: none

Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
ca. 28 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- pH: 7.38 - 7.41

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Test performed in open system: yes

SAMPLING
- Sampling frequency: every 2-4 days for at least 28 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Procedure control: yes
- Abiotic sterile control: yes
- Adsorption control: yes
- Toxicity control: yes
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
> 90 - < 100
Sampling time:
28 d
Details on results:
> 50% after 5 days
>=95% after 7, 10, 14, 17, 21, 24, 27, 28 days

BOD5 / COD results

Results with reference substance:
are valid (>=90% after 5, 7, 10, 14, 17, 21, 24, 27, 28 days)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable