6-aminonaphthalene-1,3-disulphonic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Data is from publication.

Data source

Reference

Reference Type:

publication

Title:

A Compilation Of Genotoxicity And Carcinogenicity Data On Aromatic Aminosulphonic Acids

Author:

R. Jung, D. Steinle And R. Anliker

Year:

1992

Bibliographic source:

Food and chemical toxicology Vol 30, No. 7, pp 635-660, 1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

Mutagenicity of substance Naphthalene-1, 3-disulfonic acid, 6-amino- was assessed by using Ames test.

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

6-aminonaphthalene-1,3-disulphonic acid

EC Number:

204-246-1

EC Name:

6-aminonaphthalene-1,3-disulphonic acid

Cas Number:

118-33-2

Molecular formula:

C10H9NO6S2

IUPAC Name:

6-aminonaphthalene-1,3-disulfonic acid

Test material form:

solid: crystalline

Details on test material:

- Name of test material (as cited in study report): 6 amino-1,3 naphthalene –disulfonic acid
- Molecular formula (if other than submission substance): C10H9NO6S2
- Molecular weight (if other than submission substance): 303.314g/mol
- Smiles notation (if other than submission substance): c12c(cc(S(O)(=O)=O)cc1S(O)(=O)=O)cc(N)cc2
- InChl (if other than submission substance): 1S/C10H9NO6S2/c11-7-1-2-9-6(3-7)4-8(18(12,13)14)5-10(9)19(15,16)17/h1-5H,11H2,(H,12,13,14)(H,15,16,17)
- Substance type:Organic
- Physical state:Solid

Specific details on test material used for the study:

- Name of test material:6 amino-1,3 naphthalene –disulfonic acid
- Molecular formula: C10H9NO6S2
- Molecular weight: 303.314 g/mole
- Smiles notation: c12c(cc(S(O)(=O)=O)cc1S(O)(=O)=O)cc(N)cc2
- InChl: 1S/C10H9NO6S2/c11-7-1-2-9-6(3-7)4-8(18(12,13)14)5-10(9)19(15,16)17/h1-5H,11H2,(H,12,13,14)(H,15,16,17)
- Substance type: Organic
- Physical state: Solid

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other:

Details on mammalian cell type (if applicable):

Not applicable.

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

Not specified.

Metabolic activation:

not specified

Test concentrations with justification for top dose:

Not specified.

Vehicle / solvent:

Not specified.

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

not specified

Details on test system and experimental conditions:

Not specified.

Rationale for test conditions:

Not specified.

Evaluation criteria:

Not specified.

Statistics:

Not specified.

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium, other: Not specified

Metabolic activation:

not specified

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Applicant's summary and conclusion

Conclusions:

The substance Naphthalene-1,3-disulfonic acid, 6-amino- is considered to be not mutagenic in Salmonella typhimurium.

Executive summary:

Mutagenicity of substance Naphthalene-1,3-disulfonic acid, 6-amino- was assessed by using Ames test.It shows negative mutagenic result. The substance Naphthalene-1,3-disulfonic acid, 6-amino-is considered to be not mutagenic in Salmonella typhimurium. Hence it can not be classified as gene mutant in vitro.