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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data is from Authoritive database

Data source

Referenceopen allclose all

Reference Type:
other: Authoritive database
Title:
Skin irritation for CAS NO: 91-29-2
Author:
IFA GESTIS
Year:
2017
Bibliographic source:
GESTIS SUBSTANCE Database (information system in hazardous substance of the Berufsgenossenscheftn).
Reference Type:
secondary source
Title:
Toxicological evaluations -4-Nitro-4’-aminodiphenylamine-2-sulfonic acid
Author:
BG Chemie
Year:
2000
Bibliographic source:
BG Chemie InfoCenter TOXIKOLOGISCHE BEWERTUNGEN; 06/2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Principles of method if other than guideline:
To assess the dermal irritation potential of 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonic acid in rabbits according to OECD 404
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(4-aminoanilino)-5-nitrobenzenesulphonic acid
EC Number:
202-057-9
EC Name:
2-(4-aminoanilino)-5-nitrobenzenesulphonic acid
Cas Number:
91-29-2
Molecular formula:
C12H11N3O5S
IUPAC Name:
2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonic acid
Test material form:
solid
Details on test material:
- Name of test material (as cited in study report): 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (91-29-2)
- Molecular formula: C12H11N3O5S
- Molecular weight : 309.301 g/mol
- Substance type: Organic
- Physical state: Solid

Specific details on test material used for the study:
- Name of test material (as cited in study report): 4-nitro-4’-aminodiphenylamine-2-sulfonic acid
- Molecular formula: C12H11N3O5S
- Molecular weight: 309.301 g/mol
- Substance type: Organic
- Physical state: Solid

Test animals

Species:
rabbit
Strain:
other: HC:NZW
Details on test animals or test system and environmental conditions:
- Sex: Female
- Age at study initiation: adult
- Weight at study initiation: 3.2-3.3 kg

Test system

Type of coverage:
semiocclusive
Preparation of test site:
other: Mechanically depilated skin of flanks
Vehicle:
water
Controls:
not specified
Amount / concentration applied:
500 mg




Duration of treatment / exposure:
4 hrs
Observation period:
1,24,48 and 72 hrs and day 7
Number of animals:
3 female rabbits
Details on study design:
SCORING SYSTEM: Draize method.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Time point:
24/48/72 h
Score:
0
Max. score:
4
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
mean
Time point:
24/48/72 h
Score:
0
Max. score:
4
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
None of the test animals produced skin reactions.

Applicant's summary and conclusion

Interpretation of results:
other: Not irritating
Conclusions:
The average irritation scores according to the Draize scoring for erythema and oedema were 0.0.
Hence, 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (CAS No:91-29-2) was considered to be not irritating to skin of female albino rabbits
Executive summary:

Primary Skin irritation study was conducted for test chemical 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (CAS No:91-29-2) to determine its skin irritating behavior in accordance with OECD guideline No. 404. The animals were subjected to a single 4- hour exposure to 500 mg of the test substance, mixed into a paste with water and applied semi-occlusively to the mechanically depilated skin of the flanks. The findings were scored after 1, 24, 48 and 72 hours as well as 7 days in accordance with the Draize method.

The average irritation scores according to the Draize scoring for erythema and oedema were 0.0.

Hence, 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (CAS No:91-29-2) was considered to be not irritating to skin of female albino rabbits