Sodium 2-(p-aminoanilino)-5-nitrobenzenesulphonate

Endpoint summary

Description of key information

Short-term toxicity to fish

On the basis of EPI suite, ECOSAR version 1.1, the LC50 value for short term toxicity to fish was predicted to be 111000 mg/l for sodium 2-[(4-aminophenyl)amino]-5-nitrobenzene-1-sulfonate in 96 hrs. Based on the LC50 value, it can be concluded that the substance sodium 2-[(4-aminophenyl)amino]-5-nitrobenzene-1-sulfonate as not toxic to aquatic environment and thus can be considered “not classified” per the CLP criteria.

Short-term toxicity to aquatic invertebrates

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on aquatic invertebrate predicted for sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3). Effect concentration i.e EC50 value estimated to be 144.63 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3) likely to be not toxic to aquatic invertebrates, hence it can be considered to be “ Not Classified” as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on algae was predicted sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3). Effect concentration i.e EC50 value was estimated to be 129.128 mg/l for Pseudokirchneriella for 72 hrs duration. It was concluded that the sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3) was likely to be not toxic to aquatic algae, hence it can be considered to be “not Classified” as per the CLP classification criteria for aquatic environment.

Additional information

Short-term toxicity to fish

Following study for target chemical includes predicted data from computational model developed by USEPA to conclude the

sodium 2-[(4-aminophenyl)amino]-5-nitrobenzene-1-sulfonate (CAS: 14846-08-3) toxicity extent towards fish is summarized as follows:

On the basis of EPI suite, ECOSAR version 1.1, the LC50 value for short term toxicity to fish was predicted to be 111000 mg/l for sodium 2-[(4-aminophenyl)amino]-5-nitrobenzene-1-sulfonate in 96 hrs. Based on the LC50 value, it can be concluded that the substance sodium 2-[(4-aminophenyl)amino]-5-nitrobenzene-1-sulfonate as not toxic to aquatic environment and thus can be considered “not classified” per the CLP criteria.

Short-term toxicity to aquatic invertebrates

Following four studies available for the target and read across chemical includes predicted data and experimental data to conclude tha extent of sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3) toxicity towards aquatic invertebrate are summaried below:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on aquatic invertebrate predicted for sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3). Effect concentration i.e EC50 value estimated to be 144.63 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3) likely to be not toxic to aquatic invertebrates, hence it can be considered to be “ Not Classified” as per the CLP classification criteria for aquatic environment.

Similar prediction is done by using the EPI suite, ECOSAR version 1.1, the LC50 value for short term toxicity to aquatic invertebrate was predicted to be 47889.344 mg/l for sodium 2-[(4-aminophenyl)amino]-5-nitrobenzene-1-sulfonate in 48 hrs. Based on the LC50 value, it can be concluded that the substance sodium 2-[(4-aminophenyl)amino]-5-nitrobenzene-1-sulfonate as not toxic to aquatic environment.

The above predicted data is supported by the experimental data of read across chemical 1-nitropropane (CAS: 108-03-2T) from United States Environmental Protection Agency, suggests that the EC50 effective concentration of 1-nitropropane on Daphnia manga at 24 hr is 258 mg/l. It can be concluded that the 1-nitropropane is not toxic to aquatic environment.

Further it is supported by another experimantal result of read caross chemical Disodium 4,4'-diaminostilbene-2,2'-disulphonate(CAS: 7336-20-1) from United States Environmental Protection Agency indicates that the EC50 value for Daphnia magna is > 300 and < 500 mg/l when exposed to Disodium 4,4'-diaminostilbene-2,2'-disulphonate for 48 hr. It can be concluded from the value that the Disodium 4,4'-diaminostilbene-2,2'-disulphonate is not toxic to the aquatic environment.

Thus based on theon the effect concentrations which is in the range of144.63 mg/l to 47889.344 mg/lgives the conclusion that test substance sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3) was likely to be not toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered to be“not classified”as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following four studies available for the target and read across chemical which are structurally similar to the target chemical includes predicted data and experimental data to conclude tha extent of sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3) toxicity towards algae and cyanobacteria are summaried below:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on algae was predicted sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3). Effect concentration i.e EC50 value was estimated to be 129.128 mg/l for Pseudokirchneriella for 72 hrs duration. It was concluded that the sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3) was likely to be not toxic to aquatic algae, hence it can be considered to be “not Classified” as per the CLP classification criteria for aquatic environment.

Similar prediction is done by using the EPI suite, ECOSAR version 1.1, the EC50 value for toxicity to aquatic algae was predicted to be 11619.002 mg/L mg/l for sodium 2-[(4-aminophenyl)amino]-5-nitrobenzene-1-sulfonate in 96 hrs. Based on the EC50 value, it can be concluded that the substance sodium 2-[(4-aminophenyl)amino]-5-nitrobenzene-1-sulfonate as not toxic to aquatic environment.

Above studies for target is supported by the experimental study of read across chemical Disodium 4,4'-diaminostilbene-2,2'-disulphonate (CAS: 7336-20-1) from United States Environmental Protection Agency, suggests that the The EC50 effective concentration of Selenastrum sp. Is >100 mg/L at which Growth Rate was measured when exposed to Disodium 4,4'-diaminostilbene-2,2'-disulphonate for 72 hrs. It ca be concluded form the value that the Disodium 4,4'-diaminostilbene-2,2'-disulphonate is not toxic to the aquatic environment.

Another experimental study of read across chemical 1-nitropropane ( CAS: 108-03-2) from United States Environmental Protection Agency, indicates that the 96 hour ErC50 values, based on growth rate, were greater than 456 mg a.i./L, the highest concentration tested  for Pseudokirchneriella subcapitata. It can be concluded that the 1-nitropropane is not toxic to the aquatic environment.

Thus based on the effect concentrations which is in the range >100 mg/L to 11619.002 mg/l give the conclusion that test substance sodium 2-[(4-aminophenyl)amino]-5-nitrobenzenesulfonate (Cas no. 14846-08-3) was likely to be not toxic to algae at environmentally relevant concentrations and can be considered to be“not classified”as per the CLP classification criteria.