Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
29.02.1988 - 02.03.1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study, Non-GLP
Qualifier:
according to
Guideline:
other: EU Directive 79/831/EEC, Annex V, part C
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
Preparation of test media:
The test medium of the highest test concentration, 200 mg/l (nominal) was prepared. Following this, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia
- Strain: Daphnia magna Straus
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h

ACCLIMATION
Total water hardness: 2.70 +/- 0.5 mmol/l
Acidity constant - pH 4.3: 0.80 +/- 0.1 mmol/l
Molar ratio: Ca:Mg ca. 4 :1
Oxygen content > 2 mg/l
Light conditions: 16 : 8 hours day-night regime, diffuse light (5 microeinstein/m*m*s) at 400 - 750 nm wave length
- Type and amount of food: : Whilst changing water Brewer's yeast was given, also 1 x daily grown Green algae culture
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.70 +/- 0.5 mmol/L
Test temperature:
18-20 °C
pH:
7.7-8.3
Dissolved oxygen:
9.05 - 8.6 mg/L
Nominal and measured concentrations:
Nominal concentrations:6.25, 12.5, 25, 25, 50, 100, 200 mg/L. Additionally, a control was tested in parallel.
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass tubes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates):4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: : according to the test guideline DIN 38412, L 11
pretreatment steps: tap water; 6 µm- and charcoal-filtration; H2SO4 was added to reduce alkalinity up to pH 4.3; distilled water was added to reduce water-hardness; water was aerated (oil-free air) unil saturated with oxygen; water was stored for at least 24 h for stabilization.
- Ca/mg ratio: 4:1
- Culture medium different from test medium: yes

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours day-night regime
- Light intensity: diffuse light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Visually after 0, 3, 6, 24, 48 h
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks:
not neutralized
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
39.23 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks:
not neutralized
Remarks on result:
other: 32.31 - 47.63
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks:
not neutralized
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks:
not neutralized
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
57.43 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks:
not neutralized
Remarks on result:
other: 49.79 - 66. 25

Number of mobile test animals after exposure (x h) to various test concentrations:

Concentration (mg/L)

0 h

3 h  6 h  24 h  48 h

0

20

 20  20  20  20

6.25

20

 20  20  20  20

12.5

20

 20  20  20  20

25

20

 20  20  20  16

50

20

 20  20  14  7

100

20

 5  0  0  0

200

20

 0  0  0  0

At 100 and 200 mg/L toxic effect may be due to the high pH in the test vessels (up to pH 10.57)
 

pH values after

concentration (mg/L)

0 h

48 h

0

8.07

8.0

6.25

8.89

8.23

12.5

9.25

8.26

25

9.61

8.39

50

9.95

8.81

100

10.27

9.36

200

10.57

9.45

Executive summary:

Acute toxicity to Daphnia magna has been investigated according to the methods outlined by EU Directive 79/831/EEC, Annex V, part C.

Daphnia magna were exposed for 48 hours to an aqueous solution of ethyldimethylamine at nominal concentrations of 6.25 - 12.5 - 25 - 50 - 100 and 200 mg/L.

EC 50 - 48h determined is of 39.23 mg/L and NOEC is of 12.5 mg/L.

Description of key information

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
39.23 mg/L

Additional information

Ethyldimethylamine toxicity to daphnids has been investigated by BASF (1988). The methodology followed is EU Directive 79/831/EEC, Annex V, part C. with exposure duration of 48 h. In this context, the 48 h EC50 determined is of 39.23 mg/L and the NOEC of 12.5 mg/L.