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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions, Non-GLP
Qualifier:
according to
Guideline:
other: The study was performed according to German national standard DIN 38 412, part L15 (status: Jan 1979.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Goldenorfe
- Strain: Leuciscus idus L. (golden variety)
- Source: : Fish farm Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 6.4 cm
- Weight at study initiation (mean and range, SD): 2.3 g

ACCLIMATION
- Acclimation conditions:
Culture conditions: fish were kept in oil-free aerated and charcoal filtered tap water, flow-through system
Water temperature:20 ±1 °C
pH: 7-8
- Type and amount of food: Fukosalm- Anschlussfutter 1
- Feeding frequency: ad libitum
- Health during acclimation (any mortality observed): no mortality during the last 6 weeks of housing.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
total hardness: 2.5 mmol/L
Test temperature:
20 ± 1 °C
pH:
8 ±0.1
Dissolved oxygen:
> 8 mg/L
Nominal and measured concentrations:
Nominal concentrations: 6.8, 10, 14.7, 21.5, 31.6, 46.4, 68.1, 100 mg/L. Additionally: 100 mg/L pH-adjusted
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): aquaria open
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: aeration wiht oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms
Reference substance (positive control):
yes
Remarks:
Chloracetamid
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
31.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
not neutralized
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
31.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
not neutralized
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
38.29 other: mg/L (geometric mean)
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
not neutralized
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
46.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
not neutralized
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Neutralized
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: tumbling, lateral position
Results with reference substance (positive control):
- Results with reference substance valid? yes
- LC50: 26 mg/L

Cumulative mortality

Nominal conc.(mg/l)

Number of fish

Mortality (cumulated) after

1 h

4 h

24 h

48 h

72 h

96 h

0

10

0

0

0

0

0

0

6.8

10

0

0

0

0

0

0

10.0

10

0

0

0

0

0

0

14.7

10

0

0

0

0

0

0

21.5

10

0

0

0

0

0

0

31.6

10

0

0

0

0

0

0

46.4

10

1

10

10

10

10

10

68.1

10

10

10

10

10

10

10

100

10

10

10

10

10

10

10

Symptoms

Nominal conc.(mg/l)

Number of fish

Symptoms after

4 h

0

10

6.8

10

10.0

10

14.7

10

21.5

10

31.6

10

46.4

10

Tumbling, lateral position

68.1

10

100

10

pH values

Nominal conc.(mg/l)

pH values after

1 h

24 h

48 h

72 h

96 h

0

7.4

7.6

7.6

7.6

7.6

6.8

7.9

7.8

7.7

7.7

7.8

10.0

8.6

7.6

7.6

7.6

7.7

14.7

8.8

7.8

7.7

7.8

7.8

21.5

9.3

7.6

7.5

7.6

7.6

31.6

9.5

7.8

7.7

7.7

7.7

46.4

9.8

9.3

68.1

10.1

9.3

100

10.6

9.9

Oxygen content

Nominal conc.(mg/l)

Oxygen concentrations [mg/L] after

1 h

24 h

48 h

72 h

96 h

0

8.8

8.5

8.7

8.5

8.4

6.8

9.0

9.0

9.0

8.6

8.6

10.0

8.4

8.5

8.6

8.4

8.4

14.7

8.9

8.9

8.9

8.7

8.8

21.5

8.4

8.0

8.4

8.2

8.3

31.6

8.7

8.4

8.4

8.4

8.3

46.4

8.5

9.1

68.1

9.1

9.2

100

9.1

9.2

Observed effects may be due to a pH-shift at higher test concentrations. After neutralization (100 mg/L) no effects were observed.

Validity criteria fulfilled:
not specified
Conclusions:
Ethyldimethylamine is considered harmful to fish as a worst-case.
Executive summary:

Ethyldimethylamine acute toxicity to Leuscisus idus has been investigated according to German industrial standard test guideline DIN 38412 part 15. Ethyldimethylamine acute toxicity (LC50 -96h) ranges from 38.29 to >100 mg/L depending on the neutralization state.

Description of key information

One study has been identified as relevant for characterizing ethyldimethylamine toxicity to fish. In the framework of this study carried out according to National German guidelines, the toxicity determined is to be 38.29 mg/L (LC50 -96h, pH non-adjusted). No mortality is observed up to 100 mg/L when pH was adjusted.

Key value for chemical safety assessment

LC50 for freshwater fish:
38.29 mg/L

Additional information