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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Directive 84/449/EEC, B.14
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Dimethyl malonate
EC Number:
203-597-8
EC Name:
Dimethyl malonate
Cas Number:
108-59-8
Molecular formula:
C5H8O4
IUPAC Name:
dimethyl malonate

Method

Species / strain
Species / strain / cell type:
other: S.typhimurium TA 1535, TA1537,TA1538,TA98,TA100
Metabolic activation:
with and without
Test concentrations with justification for top dose:
8 - 5000 μg/plate
Controls
Positive controls:
yes
Positive control substance:
other: Without S9
Statistics:
Mean values and standard deviation were calculated with BIOSYS software

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Remarks:
>= 1000 μg/plate
Species / strain:
S. typhimurium TA 1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Remarks:
>= 1000 μg/plate
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Remarks:
>= 1000 μg/plate
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Remarks:
>= 1000 μg/plate
Additional information on results:
The test substance did not induce a statistically significant increase in the number of revertants in any of the strains
tested, neither with nor without metabolic activation. The test substance was non-mutagenic under the conditions of this
test.
Remarks on result:
other: all strains/cell types tested Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results:
negative