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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17.10.2017 to 19.10.2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
Short term toxicity of test chemical sodium naphthalene-1-sulfonate to aquatic invertebrates was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material: sodium naphthalene-1-sulfonate
- Molecular formula: C10H7NaO3S
- Molecular weight: 230.218 g/mol
- Smiles notation: c12c(S(=O)(=O)[O-])cccc1cccc2.[Na+]
- InChl: 1S/C10H8O3S.Na/c11-14(12,13)10-7-3-5-8-4-1-2-6-9(8)10;/h1-7H,(H,11,12,13);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:
not specified
Vehicle:
yes
Details on test solutions:
The solution 100 mg/l was prepared by dissolving white powder in reconstituted water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

ACCLIMATION - No data available
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Remarks on exposure duration:
± 1 hr
Test temperature:
20±1°C
pH:
pH at higher concentration 100 mg/l: 7.7 did not change during test
Control: 7.7 change to 7.8 during test
Dissolved oxygen:
higher than 7.9 mg/L at the end of test
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).

Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.

- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.

Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:

CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
72 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 0 % Inhibition were observed at 100 mg/l
Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 0.76 mg/L (24 hours)
Reported statistics and error estimates:
EC50 was calculated using non linear regression by the software Prism 4.0
Validity criteria fulfilled:
yes
Conclusions:
After the exposure of test chemical with daphnia magna for 48 hrs, 0 % (No immobility) were observed at 100 mg/l. Thus The EC0 was observed at 100 mg/l. And it can be concluded that the EC50 was > 100 mg/l.
Executive summary:

Aim of this study was to assess the short term toxicity of sodium naphthalene-1-sulfonate to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. Limit test performed at 100 mg/l. The solution 100 mg/l was prepared by dissolving white powder in reconstituted water. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. After the exposure of test chemical with daphnia magna for 48 hrs, 0 % (No immobility) were observed at 100 mg/l. Thus The EC0 was observed at 100 mg/l. And it can be concluded that the EC50 was > 100 mg/l. Thus above result indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

Description of key information

Aim of this study was to assess the short term toxicity of sodium naphthalene-1-sulfonate to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. Limit test performed at 100 mg/l. The solution 100 mg/l was prepared by dissolving white powder in reconstituted water. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. After the exposure of test chemical with daphnia magna for 48 hrs, 0 % (No immobility) were observed at 100 mg/l. Thus The EC0 was observed at 100 mg/l. And it can be concluded that the EC50 was > 100 mg/l. Thus above result indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

Key value for chemical safety assessment

Additional information

Aim of this study was to assess the short term toxicity of sodium naphthalene-1-sulfonate to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. Limit test performed at 100 mg/l. The solution 100 mg/l was prepared by dissolving white powder in reconstituted water. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. After the exposure of test chemical with daphnia magna for 48 hrs, 0 % (No immobility) were observed at 100 mg/l. Thus The EC0 was observed at 100 mg/l. And it can be concluded that the EC50 was > 100 mg/l. Thus above result indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.