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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21.11.2017 - 25.11.2017
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical sodium naphthalene-1-sulfonate on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material: sodium naphthalene-1-sulfonate
- Molecular formula: C10H7NaO3S
- Molecular weight: 230.218 g/mol
- Smiles notation: c12c(S(=O)(=O)[O-])cccc1cccc2.[Na+]
- InChl: 1S/C10H8O3S.Na/c11-14(12,13)10-7-3-5-8-4-1-2-6-9(8)10;/h1-7H,(H,11,12,13);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:
not required
Remarks:
As the test substance was soluble in recomended condition
Vehicle:
not specified
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 100mg/L ,respectively. potable water (passed through reverse osmosis system) was used.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra Fish
- Strain:Danio rerio
- Source: Anytime Pet,Jaripatka, Nagpur
- Length at study initiation (length definition, mean, range and SD): average 1.47 cm
- Weight at study initiation (mean and range, SD): average 0.044 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod:16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
150.5 mg of CaCO3
Test temperature:
23 °C
pH:
7.2
Dissolved oxygen:
7.0 mg/l
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Nontoxic
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: No effect were observed

Fig: Mortality and symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

-

-

-

-

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical sodium naphthalene-1-sulfonate on Zebra Fish Danio rerio was determine to be > 100 mg/l. As no effect were observed at 100 mg/l.
Executive summary:

Study was conducted to access the effect of test chemical sodium naphthalene-1-sulfonate on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 100mg/L ,respectively. potable water (passed through reverse osmosis system) was used and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical sodium naphthalene-1-sulfonate on Zebra Fish Danio rerio after 96 hrs was determine to be > 100 mg/l. As no effect were observed at 100 mg/l. Based on the LC50, it can be consider that the chemical sodium naphthalene-1-sulfonate was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Study was conducted to access the effect of test chemical sodium naphthalene-1-sulfonate on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 100mg/L ,respectively. potable water (passed through reverse osmosis system) was used and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical sodium naphthalene-1-sulfonate on Zebra Fish Danio rerio after 96 hrs was determine to be > 100 mg/l. As no effect were observed at 100 mg/l. Based on the LC50, it can be consider that the chemical sodium naphthalene-1-sulfonate was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Additional information

Study was conducted to access the effect of test chemical sodium naphthalene-1-sulfonate on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 100mg/L ,respectively. potable water (passed through reverse osmosis system) was used and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical sodium naphthalene-1-sulfonate on Zebra Fish Danio rerio after 96 hrs was determine to be > 100 mg/l. As no effect were observed at 100 mg/l. Based on the LC50, it can be consider that the chemical sodium naphthalene-1-sulfonate was nontoxic and can be consider to be not classified as per the CLP classification criteria.