2,2'-phenyliminodiethanol

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Animal experimental study, predates implementation of GLP and/or development of study guidelines, limitations in design and/or reporting but otherwise adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Principles of method if other than guideline:

According to BASF-internal standard: Five to ten NMRI mice per sex per dose were exposed to the test substance dissolved in carboxymethyl cellulose (with 2-3 drops Cremophor EL) via intraperitoneal injection. Animals received 200, 250, 320, 400, 800, 1600 mg/kg bw. After an observation period of 7-14 days animals were necropsied.

GLP compliance:

no

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-phenyliminodiethanol

EC Number:

204-368-5

EC Name:

2,2'-phenyliminodiethanol

Cas Number:

120-07-0

Molecular formula:

C10H15NO2

IUPAC Name:

2-[(2-hydroxyethyl)(phenyl)amino]ethan-1-ol

Details on test material:

- Name of test material (as cited in study report): Dioxyäthyl-anilin

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

with 2-3 drops Cremophor EL

Details on exposure:

Test concentrations used were 2, 4, 8 and 16%

Doses:

200, 250, 320, 400, 800, 1600 mg/kg bw

No. of animals per sex per dose:

- 200, 400, 800 mg/kg bw: 10
- 250, 320, 1600 mg/kg bw: 5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days (all groups) or 14 days (320, 400, 800 mg/kg bw)
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 360 mg/kg bw

Mortality:

7-DAYS FOLLOW-UP
- 200 mg/kg bw: 0/10 females and 0/10 males within 7 days
- 250 mg/kg bw: 0/5 females died within 7 days. 1/5 males died within 24 hours and 1/5 within 7 days
- 320 mg/kg bw: 1/5 females died within 7 days and 0/5 males within 7 days
- 400 mg/kg bw: 1/10 females died within 48 hours, 4/10 females within 7 days. 4/10 males died within 24 hours, 2/10 within 48 hours, 1/10 within 7 days
- 800 mg/kg bw: 9/10 females died within 24 hours, the remaining female died within 48 hours. 9/10 males died within 24 hours, the remaining male died within 48 hours.
- 1600 mg/kg bw: 3/5 females died within one hour and the remaining animals died within 24 hours. 2/5 males died within one hour and 2/5 died within 24 hours.

14-DAYS FOLLOW-UP
- 320 mg/kg bw: 1/5 females and 0/5 males after 14 days
- 400 mg/kg bw: 3/5 females and 4/5 males after 14 days
- 800 mg/kg bw: 5/5 females and 5/5 males after 14 days

Clinical signs:

Dyspnea, staggering, partly atony and narcotic-like state, abdominal and dorsal position, clonic spasms, apathy were observed.

Body weight:

Normal body weight gain was observed

Gross pathology:

No intra-abdominal substance residues or adhesions were observed.