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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Animal experimental study, predates implementation of GLP and/or development of study guidelines, limitations in design and/or reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1974
Report date:
1974

Materials and methods

Principles of method if other than guideline:
According to BASF-internal standard: Five to ten NMRI mice per sex per dose were exposed to the test substance dissolved in carboxymethyl cellulose (with 2-3 drops Cremophor EL) via intraperitoneal injection. Animals received 200, 250, 320, 400, 800, 1600 mg/kg bw. After an observation period of 7-14 days animals were necropsied.
GLP compliance:
no
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-phenyliminodiethanol
EC Number:
204-368-5
EC Name:
2,2'-phenyliminodiethanol
Cas Number:
120-07-0
Molecular formula:
C10H15NO2
IUPAC Name:
2-[(2-hydroxyethyl)(phenyl)amino]ethan-1-ol
Details on test material:
- Name of test material (as cited in study report): Dioxyäthyl-anilin

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male/female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
CMC (carboxymethyl cellulose)
Remarks:
with 2-3 drops Cremophor EL
Details on exposure:
Test concentrations used were 2, 4, 8 and 16%
Doses:
200, 250, 320, 400, 800, 1600 mg/kg bw
No. of animals per sex per dose:
- 200, 400, 800 mg/kg bw: 10
- 250, 320, 1600 mg/kg bw: 5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days (all groups) or 14 days (320, 400, 800 mg/kg bw)
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 360 mg/kg bw
Mortality:
7-DAYS FOLLOW-UP
- 200 mg/kg bw: 0/10 females and 0/10 males within 7 days
- 250 mg/kg bw: 0/5 females died within 7 days. 1/5 males died within 24 hours and 1/5 within 7 days
- 320 mg/kg bw: 1/5 females died within 7 days and 0/5 males within 7 days
- 400 mg/kg bw: 1/10 females died within 48 hours, 4/10 females within 7 days. 4/10 males died within 24 hours, 2/10 within 48 hours, 1/10 within 7 days
- 800 mg/kg bw: 9/10 females died within 24 hours, the remaining female died within 48 hours. 9/10 males died within 24 hours, the remaining male died within 48 hours.
- 1600 mg/kg bw: 3/5 females died within one hour and the remaining animals died within 24 hours. 2/5 males died within one hour and 2/5 died within 24 hours.

14-DAYS FOLLOW-UP
- 320 mg/kg bw: 1/5 females and 0/5 males after 14 days
- 400 mg/kg bw: 3/5 females and 4/5 males after 14 days
- 800 mg/kg bw: 5/5 females and 5/5 males after 14 days
Clinical signs:
Dyspnea, staggering, partly atony and narcotic-like state, abdominal and dorsal position, clonic spasms, apathy were observed.
Body weight:
Normal body weight gain was observed
Gross pathology:
No intra-abdominal substance residues or adhesions were observed.

Applicant's summary and conclusion