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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Test start: 05 Dec 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Remarks:
only basic data given; legally binding classification (KBwS, WGK 2)
Qualifier:
according to
Guideline:
other: Water quality; fresh water algal growth inhibition test with Scenedesmus subspicatus and Selenastrum capricornutum
Version / remarks:
ISO 8692:1989; German version EN 28692:1993
GLP compliance:
not specified
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL- Source and lot/batch No.of test material: Merck (Name in KBWS database: N,N-Di(2hydroxyethyl)anilin)- Expiration date of the lot/batch: no data- Purity test date: no data
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: addition of 625 mg/L test item to 500 mL test medium, stirred for 0.5 h
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM - Strain: Scenedesmus subspicatus CHODAT - Source (laboratory, culture collection): laboratory culture - Age of inoculum (at test initiation): - Method of cultivation: cultured in 100-mL Erlenmeyer flasks in temperature-controlled room with uniform lighting (fluorescent tubes), pre-culture started with 7-d old algae from stock culture, pre-culture medium according to guideline
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
23± °C
pH:
stock solution (1250 mg/L): 7.26
Nominal and measured concentrations:
nominal: tested range: 156.25 to 1000 mg/L
Details on test conditions:
TEST SYSTEM - Test vessel: algae test apparatus with quasi-continuous determination of optical density (Abimed) - Initial cells density: 0.02 exteinction units = 10000 cells/mL - Control end cells density: not reported - No. of vessels per concentration (replicates): not reported - No. of vessels per control (replicates): not reported GROWTH MEDIUM - Standard medium used: yes - Detailed composition if non-standard medium was used: TEST MEDIUM / WATER PARAMETERS - Source/preparation of dilution water: test medium according to guideline OTHER TEST CONDITIONS - Sterile test conditions: yes - Adjustment of pH: no - Photoperiod: continuous - Light intensity and quality: 17 W/m² (fluorescent lamps) EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : - Determination of cell concentrations: fluorimeter (400-500 nm; emission: 685 nm) TEST CONCENTRATIONS - Spacing factor for test concentrations: - Justification for using less concentrations than requested by guideline: - Range finding study - Test concentrations: - Results used to determine the conditions for the definitive study:
Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
418 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
393 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
172 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
156 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Validity criteria fulfilled:
yes

Description of key information

With high probability acutely not harmful to algae.

Key value for chemical safety assessment

EC50 for freshwater algae:
418 mg/L
EC10 or NOEC for freshwater algae:
172 mg/L

Additional information

The assessment of the algal toxicity is based on effect data taken from the “Commission for the Evaluation of Substances Hazardous to Waters” (KBWS) data sheet no. 1385, which is part of the German Federal Water Act (Article 19g). Based on the collected data a substance is classified according to its water-hazardous properties (WGK 1 to 3). 2,2 -phenyliminodiethanol was classified as WGK 2, which indicates a hazard to waters. As the list with the classified substances was published in the Bundesanzeiger (Federal Ministry of Justice, 2005. Bundesanzeiger No. 142 a),

the classification is legally binding for the industry.

 

The KBWS contains an abstract on the algae test according to DIN EN 28692 (= ISO 8692). The test species was Desmodesmus subspicatus. Test item concentrations were not analytically verified, but can be assumed to be stable based on the analytical results from an acute toxicity study to daphnids (BASF AG, 1998). The 72 -h ErC50 was determined to be 418 mg/L based on growth rate (72 -h EbC50 = 393 mg/L). The EC10 was only determined for biomass (72 -h EbC10 = 172 mg/L; KBWS, 1995). As the endpoint biomass is generally somewhat lower than the growth rate and can therefore be considered as a conservative value.

Based on the available data, 2,2 -phenyliminodiethanol is assessed to be not acutely harmful to algae with high probability. Chronic effects are not expected as well.