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Ecotoxicological information

Toxicity to microorganisms

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Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: DIN 38404 part 2
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Purity: 99.55 %
Batch: 583021
Analytical monitoring:
not specified
Vehicle:
yes
Remarks:
Tween 80
Details on test solutions:
The test item was dissolved in distilled water containing 0.01 % Tween 80.
Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Source: Berlin 33/2 from DSM, Göttingen, Germany
- Cultured on inclined agar tubes acroding to DIN 38412.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h
Test temperature:
21 °C
Salinity:
NA
Nominal and measured concentrations:
0, 62.5, 125, 250, 500, 1000 and 2000 mg/L
Details on test conditions:
500 mg of the active substance were added to 200 mL aqua dest. (containing 0.01% Tween 80) and shaken for 24 hours at 21 °C, 190 rpm and pH > 11 (adjusted with 1 N NaOH). Then the solution was adjusted with 1 N HCl to pH 7.84. Al test batched were placed in a shaking incubator and incubated for 17 ± 1 hours at 21 ± 1 °C and 180 rpm.
Reference substance (positive control):
no
Key result
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
170 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
17 h
Dose descriptor:
NOEC
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Determination of % inhibition

Concentration, mg/L

% inhibition

62.5

125

250

500

1000

2000

32.0

45.8

50.9

67.2

85.2

95.3

 

Validity criteria fulfilled:
not specified
Remarks:
expected to be valid source
Conclusions:
For MCPP-P acid, the hydrolysis product of MCPP-P 2-EHE, a 17h-EC50 of 170 mg/L, 17h-EC10 of 20 mg/L and a 17h-NOEC of 12 mg/L were determined on Pseudomonas putida.
Executive summary:

In a static test system according to DIN 38412 part 8 for MCPP-P acid a 17h-EC50 of 170 mg/L and a 17h-NOEC of 12 mg/L was observed. The test concentrations were 0, 62.5, 125, 250, 500, 1000 and 2000 mg/L dissolved in distilled water containing 0.001 % Tween 80 with 6 batches per concentration. The test batches were placed in a shaking incubator and incubated for 17 hours at 21 °C. The inhibition on bacterial growth was determined by calculations based on the cell densities measured by photometer.

 

Endpoint:
toxicity to microorganisms, other
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Please refer to section 13 for "Read Across justification".
Reason / purpose for cross-reference:
read-across source
Key result
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
170 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
17 h
Dose descriptor:
NOEC
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Description of key information

For MCPP-P acid, the hydrolysis product of MCPP-P 2-EHE, a 17h-EC50 of 170 mg/L, 17h-EC10 of 20 mg/L and a 17h-NOEC of 12 mg/L were determined on Pseudomonas putida.

Key value for chemical safety assessment

EC50 for microorganisms:
170 mg/L
EC10 or NOEC for microorganisms:
20 mg/L

Additional information

The toxicity to microorganisms was investigated with MCPP-P acid (read-across).

In a static test system according to DIN 38412 part 8 for MCPP-P acid a 17h-EC50 of 170 mg/L and a 17h-NOEC of 12 mg/L was observed. The test concentrations were 0, 62.5, 125, 250, 500, 1000 and 2000 mg/L dissolved in distilled water containing 0.001 % Tween 80 with 6 batches per concentration. The test batches were placed in a shaking incubator and incubated for 17 hours at 21 °C. The inhibition on bacterial growth was determined by calculations based on the cell densities measured by photometer.