2-chloro-2-methylbutane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

unsuitable test system

Remarks:

and documentation insufficient for assessment.

Data source

Reference

Reference Type:

publication

Title:

Acute Lethal Toxicity of Environmental Pollutants to Aquatic Organisms.

Author:

Yen J-H, Lin K-H, Wang Y-S

Year:

2002

Bibliographic source:

Ecotoxicology and Environmental Safety 52 (2), 113-116.

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test: Toxicity on dahpnie was determined by testing several haloalkanes.
- Short description of test conditions: 20 daphnia were tested in 100 mL test solution with six different concentrations at 25 +/- 1°C, static exposure.
- Parameters analysed / observed: After an incubation period of 4 h, mortality was observed. If the concentration of the test solution for mortality was beyond the test range, the test was repeated with a more appropriate concentration.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chloro-2-methylbutane

EC Number:

209-836-2

EC Name:

2-chloro-2-methylbutane

Cas Number:

594-36-5

Molecular formula:

C5H11Cl

IUPAC Name:

2-chloro-2-methylbutane

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Daphnia pulex

Details on test organisms:

Daphnia (Daphnia pulex), obtained from Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Taichung, Taiwan
The daphnia were acclimatized for 2 weeks under the same conditions as in the test. In the test, daphnia 24 h after hatching were used.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

4 h

Test conditions

Hardness:

215 mg/L CaCO3

Test temperature:

25+/-1 °C

pH:

6.6

Dissolved oxygen:

DO 4.9 mg/L

Nominal and measured concentrations:

0, 50, 100, 200, 500, 1000 µg/mL were tested

Details on test conditions:

no further details specified

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

4 h

Dose descriptor:

EC50

Effect conc.:

2.37 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Remarks:

no guideline followed