1-ethoxypropan-2-ol

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Recent study, well reported in open literature.

Data source

Reference

Reference Type:

publication

Title:

Potential of the octanol-water partition coefficient (logP) to predict the dermal penetration behaviour of amphiphilic compounds in aqueous solutions.

Author:

Korinth G, Wellner T, Schaller KH, Drexler H

Year:

2012

Bibliographic source:

Toxicology Letters 125, 49-53

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 428 (Skin Absorption: In Vitro Method)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1-ethoxypropan-2-ol

EC Number:

216-374-5

EC Name:

1-ethoxypropan-2-ol

Cas Number:

1569-02-4

Molecular formula:

C5H12O2

IUPAC Name:

1-ethoxypropan-2-ol

Details on test material:

Source: Hydro (formerly Vereinigte Aluminium Werke, Grevenbroich, Germany)
Purity: >98%.

Administration / exposure

Vehicle:

other: 50% in 0.9% NaCl in water.

Details on in vitro test system (if applicable):

SKIN PREPARATION
- Source of skin: Local hospital
- Ethical approval if human skin: no information.
- Type of skin: Human abdomen removed following reductive plastic surgery.
- Preparative technique: The skin was separated from subcutaneous fat tissue, wrapped in aluminium foil, and stored in plastic bags. Prior to experiments, the skin was thawed at room temperature.
- Thickness of skin (in mm): 0.9mm
- Membrane integrity check: The integrity of skin membranes was controlled visually and by measurement of skin surface temperatures using a digital quick response precision thermometer with a surface measuring probe.
- Storage conditions: −20C for up to 8 months.

PRINCIPLES OF ASSAY
- Diffusion cell: 0.64cm2
- Receptor fluid: 0.9% NaCl solution in water, stirred continuously.
- Solubility of test substance in receptor fluid: fully miscible.
- Static system: yes
- Test temperature: 37C
- Occlusion: yes
- Reference substance(s):
- Other: Substance was applied as neat liquids or as 50% (v/v) aqueous solutions under infinite dose. The receptor fluid was stirred continuously, and samples were collected 8 h after compound exposure. Each sampled volume was immediately replaced with fresh receptor fluid. Samples were deep frozen and analysed within several days. Detection was was accomplished by solid-phase extraction and subsequent GC/FID analysis.

Results and discussion

Percutaneous absorption

Percutaneous absorption 1

Remarks on result:

other: Average flux

Remarks:

neat compound: 1389±400ug/cm2/hr

Percutaneous absorption 2

Remarks on result:

other: Average flux

Remarks:

50% solution: 2133±101ug/cm2/hr

Applicant's summary and conclusion

Executive summary:

In a study designed to generate data to confirm or disprove a hypothesis that rates of dermal penetration can be predicted from the octanol water partion coefficient, the rate of 1 -ethoxyypropano-2 -ol penetration in vitro through human skin was determined. The rate of flux of neat substance was quite high at ~1400 ±400ug/cm2/hr (similar to the level measured for ethanol) and increased by around 50% to around 2100±100ug/cm2/hr in the presence of water (50% aqueous solution).