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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours. 
The study was conducted under static conditions with an initial cell density of 4332 cells/mL. Five water accommodated fractions (WAF) were prepared with a nominal loading of 10.0 - 20.0 - 40.0 - 80.0 - 160 mg test item/L and removal of particulate matter by sedimentation. Three replicates were used for each water accommodated fraction and six for the control. The environmental conditions were within the acceptable limits. All test loadings and the control were analytically verified via analysis of dissolved organic carbon (DOC, according to DIN EN 1484) measured as NPOC(Non Purgeable Organic Carbon) at the start and the end of the exposure. All effect values given are based on nominal test item loadings of the test item.

Key value for chemical safety assessment

EC50 for freshwater algae:
160 mg/L
EC10 or NOEC for freshwater algae:
40 mg/L

Additional information

In this study inhibition on growth of the freshwater green alga Desmodesmus subspicatus was found after 72 hours exposure to the test item. The nominal EL50-values for inhibition of growth rate (ErL50) after 72 hours was > 160 mg/L. The nominal NOEL-values for inhibition of growth rate after 72 hours was 40.0 mg/L. All effect values given are based on nominal test item loadings. The key value reads as follows: ErL50 > 160mg/L.