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EC number: 689-267-8 | CAS number: 501925-98-0
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the Water Accommodated Fraction (WAF) with the nominal loading of 100 mg/L of the test item were determined according to OECD 202 (2004) and Council Regulation (EC) No. 440/2008 Method C.2 (2008) over a period of 48 hours. 100 mg/L of the test item were shaken with dilution water at 20 rpm for 24 hours at room temperature . After a 1-h settlement period, the test item phase was separated by siphon and the test organisms exposed to the clear aqueous phase. Twenty daphnids were exposed to the WAF and the control.The tested WAF with a nominal loading of 100 mg/L of the test item and the control were analytically verified by analysis of dissolved organic carbon at the start of the exposure (0 h) and at the end of the exposure (48 h). The measured concentrations of dissolved organic carbon in the control were < 2.00 mg C/L. The measured concentrations of dissolved organic carbon in the WAF with the nominal loading of 100 mg/L were 38.1 mg C/L (0 h) and 40.1 mg C/L (48 h). Exposures are expressed in terms of the original concentrations of the test item in water at the start of the mixing period (loading rate). Water quality parameters measured at the start (0 h) and at the end of the exposure (48 h), were within the acceptable limits. The validity criteria of the test guidelines were fulfilled.
At the water accommodated fraction (WAF) with a nominal loading of 100 mg/L of the test item no effects were observed on Daphnia magna. The key value reads as follows: EL 50 > 100 mg/L.
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