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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The ready biodegradability of the test item was determined with non-adapted activated sludge in the Manometric Respirometry Test for a period of 28 days according to OECD guideline 301 F .The test item concentration selected as appropriate was 30 mg/L, corresponding to a ThOD of 51 mg O2/L per test vessel.
The mean oxygen depletion in the inoculum control was 9.9 mg O2/L on day 28. The pass level > 60 % was reached by the functional control within 2 days. The biodegradation reached a maximum of 100 % degradation on day 27. In the toxicity control containing both test and reference item 64 % degradation occurred within 14 days with a maximum of 68% after 28 days. The test item does not inhibit the bacteria in the sewage sludge.
Both test item replicates reached the 10 % level (beginning of biodegradation) within 1 day. The 60 % pass level was not reached within 28 days by the test item replicates. After 28 days the mean biodegradation was 40 %.
 
The validity criteria of the guideline are fulfilled.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Both test item replicates reached the 10 % level (beginning of biodegradation) within 1 day. The 60 % pass level was not reached within 28 days by the test item replicates. After 28 days the mean biodegradation was 40 %. The test item is classified as not readily biodegradable within 28 days.