Reaction products of slack wax (petroleum), oxidized, esterified with n-butanol

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05 October 2015 to 16 November 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline Study

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Radiolabelling:

no

Test temperature:

30 °C

Details on study design: HPLC method:

EQUIPMENT
- Test utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.

DETERMINATION OF DEAD TIME
- Method: The dead time was determined by measuring the retention time of formamide (purity: 99.94%) at 680 mg/L in methanol..

REFERENCE SUBSTANCES
- Solutions of reference standards were prepared in methanol (see table 3.1)

PREPARATION OF THE SAMPLE SOLUTION
- Test item (0.1039 g) was diluted to 10 mL with tetrahydrofuran

DETERMINATION OF RETENTION TIMES
- The sample, dead time and reference standard solutions were injected in duplicate using the following high performance liquid chromatography (HPLC) parameters:
HPLC system : Agilent Technologies 1200, incorporating workstation and autosampler
Detectors: Agilent variable wavelength detector (VWD) and Polymer Laboratories evaporative light scattering detector 2100 (ELSD)
Column : Water XSelect CN 5 µm (150 x 4.6 mm id)
Column Temperature : 30 °C
Flow rate : 1.0 mL/min
Mobile phase : methanol: purified water (55:45 v/v)
pH of mobile phase : 6.8
Injection volume : 10 μL
UV detector wavelength (dead time and reference standards): 210 nm
ELSD parameters (sample): nebuliser temperature: 40 °C; evaporator temperature: 80 °C; gas flow: 1.0 L/min

The mobile phase was ramped to 100% tetrahydrofuran shortly after the elution of the last reference standard to elute the highly retained test item components. This was carried out for the sample and sample blank injections only.

EVALUATION
- Calculation of capacity factors k':
The capacity factors were determined using the following equation:
k’ = (tr– t0)/ t0
where:
k’: capacity factor; tr: retention time (min); t0: dead time (min)

Construction of the calibration curve:
A correlation of log10 k’ versus Log10Koc of the calibration standards was plotted using linear regression. The capacity factor (k’) for the reference standards was calculated from the retention time data of the dead time and reference standard solutions. Log10Koc values of the reference standards are those quoted in OECD 121.

- Determination of the log Koc value:
Adsorption Coefficient:
Log10Koc = (Log10k’-A) /B

Koc: adsorption coefficient
k’: capacity factor
A: intercept of the calibration curve
B: slope of the calibration curve

Type:

Koc

Remarks on result:

other: >4.27x10E05

Key result

Type:

log Koc

Value:

> 5.63

Temp.:

30 °C

Details on results (HPLC method):

- Typical chromatograms are attached.
- Retention times of the dead time and the retention times, capacity factors and log10 Koc values for the reference standards are shown in Tables 3.2 and 3.3 (attached).
- The calibration curve is shown in Figure 3.1 (attached).
- The retention times, capacity factor and log10 Koc value determined for the sample are shown in Table 3.4 (attached).

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient of the test item has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63.

Executive summary:

GUIDELINE

The determination was carried out using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001, and Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008.

METHOD

The test utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.

RESULTS

The adsorption coefficient of the test item has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63.

Description of key information

The adsorption coefficient of the test item has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63 (OECD 121 and EU Method C.19).

Key value for chemical safety assessment

Koc at 20 °C:

426 580

Additional information

Adsorption / desorption

GUIDELINE

The determination was carried out using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001, and Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008.

METHOD

The test utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.

RESULTS

The adsorption coefficient of the test item has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63.

[LogKoc: 5.63]