Formaldehyde, oligomeric reaction products with phenol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as mention below

Principles of method if other than guideline:

The objective of this study was to determine the effect of test chemical on the oxygen consumption rate which was associated with the peptone degradation of mixed bacterial culture.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

Details on test solutions
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: standard sock solution were prepared in distilled water according to the standard method. Appropriate dilutions of industrial wastewater were then prepared using water from the source of the receiving stream.
Other: Test chemical present in the wastewater obtain from the site of chemical manufacturing industry.

Test organisms (species):

other: Mixed bacterial culture

Details on inoculum:

The culture was obtained from the settled nontoxic municipal wastewater.

Test type:

not specified

Water media type:

not specified

Total exposure duration:

120 h

Test temperature:

20 ± 1 °C

pH:

7.8

Reference substance (positive control):

yes

Remarks:

Phenol and formaldehyde

Key result

Duration:

120 h

Dose descriptor:

other: EC35

Effect conc.:

100 other: v/v%

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: O2 consumption

Remarks on result:

other: 35% inhibition was observed

Details on results:

Study was conducted to determine effective concentration of test chemical. Based on the effect of test chemical on oxygen production and consumption rates of bacteria isolated from settled nontoxic municipal wastewater, the EC34 was determine to be100 v/v%.

Reported statistics and error estimates:

Effective concentrations (EC10, EC50) for bacteria were calculated using linear regression analysis (APHA-AWWA-WEF)

Table: Toxicity of phenol and formaldehyde to mixed bacterial culture

Scenedesmus quadricauda	Phenol (mg/L)	Formaldehyde (mg/L)
120 h EC10	283	14.7
120 h EC50	510	34.1
120 h EC90	921	78.9

Validity criteria fulfilled:

not specified

Conclusions:

Study was conducted to determine effective concentration of test chemical. Based on the effect of test chemical on oxygen production and consumption rates of bacteria isolated from settled nontoxic municipal wastewater, the EC34 was determine to be100 v/v%.

Executive summary:

Aim of this study was to determine the effect of test chemical on the oxygen consumption rate which was associated with the peptone degradation of mixed bacterial culture. Test chemical present in the wastewater obtain from the site of chemical manufacturing industry. Standard sock solution were prepared in distilled water according to the standard method. Appropriate dilutions of industrial wastewater were then prepared using water from the source of the receiving stream. The pH and dissolved oxygen were measured daily. The oxygen production and consumption rates were measured on Warburg apparatus and the effective concentrations (EC10, EC50) for bacteria were calculated using linear regression analysis. Study also involves the finding out whether the effect of pure phenol and formaldehyde to aquatic organisms was similar to that produced by the mixture of both in the wastewater. Mixed bacterial culture was obtained from the settled nontoxic municipal wastewater. Based on the 35 % oxygen consumption rate which was associated with the peptone degradation of mixed bacterial culture in 120 hrs by the test material, the EC35 was determined to be 100 v/v%. As chemical was readily degrade thus we concluded that chemical was consider as nontoxic.

Description of key information

Study was conducted to determine effective concentration of test chemical. Based on the effect of test chemical on oxygen production and consumption rates of bacteria isolated from settled nontoxic municipal wastewater, the EC34 was determine to be100 v/v%.

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L

Additional information

Aim of this study was to determine the effect of test chemical on the oxygen consumption rate which was associated with the peptone degradation of mixed bacterial culture. Test chemical present in the wastewater obtain from the site of chemical manufacturing industry. Standard sock solution were prepared in distilled water according to the standard method. Appropriate dilutions of industrial wastewater were then prepared using water from the source of the receiving stream. The pH and dissolved oxygen were measured daily. The oxygen production and consumption rates were measured on Warburg apparatus and the effective concentrations (EC10, EC50) for bacteria were calculated using linear regression analysis. Study also involves the finding out whether the effect of pure phenol and formaldehyde to aquatic organisms was similar to that produced by the mixture of both in the wastewater. Mixed bacterial culture was obtained from the settled nontoxic municipal wastewater. Based on the 35 % oxygen consumption rate which was associated with the peptone degradation of mixed bacterial culture in 120 hrs by the test material, the EC35 was determined to be 100 v/v%. As chemical was readily degrade thus we concluded that chemical was consider as nontoxic.