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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
experimental data of read across substances
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two toxicity to micro-organism studies as-
2. and 3.
GLP compliance:
not specified
Analytical monitoring:
no
Remarks:
2. no and 3. not specified
Details on sampling:
2.
- Concentrations: 8 different nominal concentrations of test chemical were used. Test chemical conc. ranges from 0.058 to 3.2 mg/l, respectively.
- Sampling method: Stock solution of test chemical was prepared with DMSO (100 mg/l).

3. Details not known
Vehicle:
yes
Remarks:
2. DMSO (100 mg/l) was used as a vehicle and 3. not specified
Test organisms (species):
other: 2. Selenastrum capricornutum and 3. Scenedesmus subspicatus
Details on test organisms:
2.
TEST ORGANISM
- Common name: Green algae
- Strain: ATCC 22662

3.
TEST ORGANISM
- Common name: Green algae
Test type:
static
Water media type:
freshwater
Total exposure duration:
72 h
Details on test conditions:
2. TEST SYSTEM
- Type (delete if not applicable): open

3. Details not known
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 2. p < 0.05
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 2.
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 3.
Validity criteria fulfilled:
not specified
Conclusions:
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value the test chemical can be expected to be ranges from 0.25 to 0.6 mg/l, respectively.
Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on aquatic algae and cyanobacteria.The studies are as mentioned below:

Short term toxicity to aquatic algae study was carried out for 72 hrsfor assessing the effect of test chemical on Selenastrum capricornutum. The study was performed in accordance with the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static fresh water system. Selenastrum capricornutum ATCC 22662 (Green algae) was used as test organism.8 different nominal concentrations of test chemical were used. Test chemical conc. ranges from 0.058 to 3.2 mg/l, respectively. Stock solution of test chemical was prepared with DMSO (100 mg/l). No analytical monitoring of test chemical concentration were done. On the basis of effect of the test chemical on biomass of the test organism Selenastrum capricornutum, the 72 hr NOEC and EC50 value was determined to be 0.10 and 0.25 mg/l, respectively.

For the test chemical, short term toxicity to aquatic algae study was carried out for 72 hrs for assessing the effect of test chemical on Scenedesmus subspicatus. The study was performed in accordance with the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static fresh water system. Scenedesmus subspicatus (Green algae) was used as test organism. On the basis of effect of the test chemical on growth rate of the test organism Scenedesmus subspicatus, the 72 hr ErC50 value was determined to be 0.6 mg/l.

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value the test chemical can be expected to be ranges from 0.25 to 0.6 mg/l, respectively. Thus, based on the EC50 value, test chemical can be considered as toxic to aq. algae and thus can be considered to be classified in ‘Aquatic acute 1 category’ as per CLP classification criteria.

Description of key information

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value the test chemical can be expected to be ranges from 0.25 to 0.6 mg/l, respectively.

Key value for chemical safety assessment

EC50 for freshwater algae:
0.25 mg/L

Additional information

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on aquatic algae and cyanobacteria.The studies are as mentioned below:

 

Short term toxicity to aquatic algae study was carried out for 72 hrsfor assessing the effect of test chemical on Selenastrum capricornutum. The study was performed in accordance with the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static fresh water system. Selenastrum capricornutum ATCC 22662 (Green algae) was used as test organism.8 different nominal concentrations of test chemical were used. Test chemical conc. ranges from 0.058 to 3.2 mg/l, respectively. Stock solution of test chemical was prepared with DMSO (100 mg/l). No analytical monitoring of test chemical concentration were done. On the basis of effect of the test chemical on biomass of the test organism Selenastrum capricornutum, the 72 hr NOEC and EC50 value was determined to be 0.10 and 0.25 mg/l, respectively.

 

For the test chemical, short term toxicity to aquatic algae study was carried out for 72 hrs for assessing the effect of test chemical on Scenedesmus subspicatus. The study was performed in accordance with the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static fresh water system. Scenedesmus subspicatus (Green algae) was used as test organism. On the basis of effect of the test chemical on growth rate of the test organism Scenedesmus subspicatus, the 72 hr ErC50 value was determined to be 0.6 mg/l.

 

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value the test chemical can be expected to be ranges from 0.25 to 0.6 mg/l, respectively. Thus, based on the EC50 value, test chemical can be considered as toxic to aq. algae and thus can be considered to be classified in ‘Aquatic acute 1 category’ as per CLP classification criteria.