Registration Dossier
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EC number: 202-336-5 | CAS number: 94-47-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of test chemical
- Justification for type of information:
- Experimental data of test chemical is from handbook and collection of data
- Reason / purpose:
- read-across source
- Reason / purpose:
- read-across source
- Qualifier:
- according to
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- WoE was prepared on the basis of toxicity of test chemical on microorganisms including
WoE 1 and WoE 2 - GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Test organisms (species):
- other:
- Test type:
- other: 1. Photobacterium leiognathi SB strain, 2. Tetrahymena thermophila
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- 24 hrs study was also included and measured
- Post exposure observation period:
- 1. Luminescence inhibition of the naturally luminescent marine bacteria Photobacterium leiognathi SB strain was measured after 30 min of exposure duration.
2. Measurement of growth inhibition of test organism was carried out at 440 nm at 24 and 28 hrs. - Details on test conditions:
- 1. EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Bacterial luminescence inhibition was measured after 30 mins of exposure duration to tested solutions.
2. EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Measurement of growth inhibition of test organism was carried out at 440 nm at 24 and 28 hrs. - Reference substance (positive control):
- not required
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Decrease in bacterial light production
- Remarks on result:
- other: 1st study
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 2nd study
- Validity criteria fulfilled:
- not specified
- Conclusions:
- 1. Based on decrease in bacterial light production by the test organism Photobacterium leiognathi SB strain, the EC50 value after 15 and 30 exposure duration with the test chemical, EC50 was observed to be 1.3 and 1.6 mg/l, respectively and LOEC value was observed to be 0.25 mg/l.
2. Toxicity to micro-organisms study was conducted on Tetrahymena thermophila for 28 hrs. Protoxkit bioassay test was performed for the toxicity study.
Thus based on both studies chemical toxicity value ranges from 0.48 to 1.6 mg/l. - Executive summary:
Data from various test chemicals was reviewed to determine the toxic nature of the test chemical. The studies are as mentioned below:
In the first study toxicity to micro-organisms study was conducted on Photobacterium leiognathi SB strain for 30 mins. Toxscreen bioassay test was performed for the toxicity study. All stock solutions were prepared in methanol and serially diluted in distilled water to obtain the target concentrations. The methanol concentration in the exposure solutions, including controls, was 0.01% (v/v) in distillate water in the tested solutions. 2-4-dichlorophenol (DCP) was used as control solution. The test bacteria was exposed to the test substance with exposure duration of 15 and 30 mins. Bacterial luminescence inhibition was measured after 15 and 30 mins of exposure to tested solutions. Based on decrease in bacterial light production by the test organism Photobacterium leiognathi SB strain, the EC50 value after 15 and 30 exposure duration with the test chemical, EC50 was observed to be 1.3 and 1.6 mg/l, respectively and LOEC value was observed to be 0.25 mg/l.
Similar All stock solutions were prepared in methanol and serially diluted in distilled water to obtain the target concentrations. The methanol concentration in the exposure solutions, including controls, was 0.01% (v/v) in distillate water in the tested solutions. Potassium dichromate (K2Cr2O7) was used as control substance. The test organism was exposed to the test substance with exposure duration 28 hrs. Inhibition in bacterial growth was measured after 24 and 28 hrs of exposure to tested solutions. Based on growth rate inhibition of test organism Tetrahymena thermophila, the EC50 value after 24 and 28 hrs exposure duration was observed to be 4.3 and 5.7 mg/l, respectively and LOEC value ewas determine to be 0.48 mg/l.
Thus based on both studies chemical toxicity value ranges from 0.48 to 1.6 mg/l.
Reference
Table: Toxicity of the test compound in ToxScreen bioassay test.
Chemical | ToxScreen bioassay test | ||
EC50 (15 min) | EC50 (30 min) | LOEC | |
Test chemical |
1.3 ± 0.1 |
1.6 ± 0.3 |
0.25 |
Description of key information
1. Based on decrease in bacterial light production by the test organism Photobacterium leiognathi SB strain, the EC50 value after 15 and 30 exposure duration with the test chemical, EC50 was observed to be 1.3 and 1.6 mg/l, respectively and LOEC value was observed to be 0.25 mg/l.
2. Toxicity to micro-organisms study was conducted on Tetrahymena thermophila for 28 hrs. Protoxkit bioassay test was performed for the toxicity study.
Thus based on both studies chemical toxicity value ranges from 0.48 to 1.6 mg/l.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 0.48 mg/L
Additional information
Data from various test chemicals was reviewed to determine the toxic nature of the test chemical. The studies are as mentioned below:
In the first study toxicity to micro-organisms study was conducted on Photobacterium leiognathi SB strain for 30 mins. Toxscreen bioassay test was performed for the toxicity study. All stock solutions were prepared in methanol and serially diluted in distilled water to obtain the target concentrations. The methanol concentration in the exposure solutions, including controls, was 0.01% (v/v) in distillate water in the tested solutions. 2-4-dichlorophenol (DCP) was used as control solution. The test bacteria was exposed to the test substance with exposure duration of 15 and 30 mins. Bacterial luminescence inhibition was measured after 15 and 30 mins of exposure to tested solutions. Based on decrease in bacterial light production by the test organism Photobacterium leiognathi SB strain, the EC50 value after 15 and 30 exposure duration with the test chemical, EC50 was observed to be 1.3 and 1.6 mg/l, respectively and LOEC value was observed to be 0.25 mg/l.
Similar All stock solutions were prepared in methanol and serially diluted in distilled water to obtain the target concentrations. The methanol concentration in the exposure solutions, including controls, was 0.01% (v/v) in distillate water in the tested solutions. Potassium dichromate (K2Cr2O7) was used as control substance. The test organism was exposed to the test substance with exposure duration 28 hrs. Inhibition in bacterial growth was measured after 24 and 28 hrs of exposure to tested solutions. Based on growth rate inhibition of test organism Tetrahymena thermophila, the EC50 value after 24 and 28 hrs exposure duration was observed to be 4.3 and 5.7 mg/l, respectively and LOEC value ewas determine to be 0.48 mg/l.
Thus based on both studies chemical toxicity value ranges from 0.48 to 1.6 mg/l.
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