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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
Acute Immobilization study of was conducted on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.
GLP compliance:
no
Analytical monitoring:
yes
Vehicle:
yes
Details on test solutions:
The test substance was partially soluble in water having solubility in DI water was 21.6523 mg/L. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 L of ADaM’s media. Kept it for 72 hrs stirring , after stirring stock was analytically detected, the solubility of stock in adams media found to be 316.102 mg/L. Used thses stock for further test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
24 and 48 hrs
Hardness:
155.5 mg of CaCO3
Test temperature:
20 ± 2
pH:
7.4 to 7.9
Nominal and measured concentrations:
6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Toxic

9.1. Immobility

Immobility

Nominal

Number of Daphnids

Immobility

 

 

 

Concentration

0 h

24 h

48 h

(mg/l)

 

 

 

 

Control

10

-

-

-

6.25

10

-

-

5

12.5

10

-

10

10

25

10

-

10

10

50

10

-

10

10

100

10

-

10

10

pH and oxygen content:

9.2. pH and oxygen content:

 

 

 

Nominal Concentration Number of

pH(mg/L)

 

(mg/l)

daphnids

0h

48h

Control

10

7.4

7.5

6.25

10

7.5

7.7

12.5

10

7.5

-

25

10

7.5

-

50

10

7.7

-

100

10

7.9

-

Nominal Concentration Number of

DO(mg/L)

 

(mg/l)

daphnids

0h

48h

Control

10

7.0

6.8

6.25

10

7.0

6.6

12.5

10

7.0

-

25

10

7.0

-

50

10

7.0

-

100

10

7.0

-

9.3. Test temperature

 

 

 

Nominal Concentration Number of

Temparature

(mg/l)

daphnids

0h

48h

Control

10

21.9

22.1

6.25

10

21.9

22.1

12.5

10

21.9

-

25

10

21.9

-

50

10

21.9

-

100

10

21.9

-

 

 

 

Validity criteria fulfilled:
yes
Remarks:
1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
Conclusions:
Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be 6.25 mg/l.
Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test chemical. The test substance was partially soluble in water having solubility in DI water was 21.6523 mg/L. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 L of ADaM’s media. Kept it for 72 hrs stirring , after stirring stock was analytically detected, the solubility of stock in adams media found to be 316.102 mg/L.Used thses stock for further test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test chemical on Daphnia magna in a 48 hours study on the basis of immobilization effect was observed to be 6.25 mg/l. Test consider to be valid because it meets the criteria as per the OECD guideline. First in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. Secondly the dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, does exhibit short term toxicity to Daphnia and consider to be toxic and classified as aquatic chronic 2 as per the CLP classification criteria.

Description of key information

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test chemical. The test substance was partially soluble in water having solubility in DI water was 21.6523 mg/L. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 L of ADaM’s media. Kept it for 72 hrs stirring , after stirring stock was analytically detected, the solubility of stock in adams media found to be 316.102 mg/L.Used thses stock for further test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test chemical on Daphnia magna in a 48 hours study on the basis of immobilization effect was observed to be 6.25 mg/l. Test consider to be valid because it meets the criteria as per the OECD guideline. First in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. Secondly the dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, does exhibit short term toxicity to Daphnia and consider to be toxic and classified as aquatic chronic 2 as per the CLP classification criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
6.25 mg/L

Additional information

Various short term studies available for the test chemical were reviewed to determine the toxic nature of test chemical on the growth and mobility of aquatic invertebrates. The studies are as mentioned below:

 

In the first key study Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test chemical. The test substance was partially soluble in water having solubility in DI water was 21.6523 mg/L. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 L of ADaM’s media. Kept it for 72 hrs stirring , after stirring stock was analytically detected, the solubility of stock in adams media found to be 316.102 mg/L.Used thses stock for further test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test chemical on Daphnia magna in a 48 hours study on the basis of immobilization effect was observed to be 6.25 mg/l. Test consider to be valid because it meets the criteria as per the OECD guideline. First in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. Secondly the dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, does exhibit short term toxicity to Daphnia and consider to be toxic and classified as aquatic chronic 2 as per the CLP classification criteria.

 

Above study was supported by the second study. Determination of the inhibition of the mobility of daphnids was carried out with the test substance according to OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving colourless liquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 1.2, 2, 3.5, 5.8 and 10 mg/l under the static system. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 3.6 mg/L on the basis of mobiity inhibition effects in a 48 hour study. This value indicates that the test chemical is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 2 category as per the CLP criteria.

Thus based on the above effects and studies available on the aquatic invertebrate, test chemical consider to be toxic to the aquatic invertebrates and hence classified in aquatic chronic 2 category as per the CLP classification criteria.