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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
January 03 to May 18, 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Version / remarks:
1992
Qualifier:
according to guideline
Guideline:
EPA OPPTS 835.3110 (Ready Biodegradability)
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
ethyl 2-cyclopentylidene-3-oxobutanoate
EC Number:
812-640-5
Cas Number:
95296-11-0
Molecular formula:
C11H16O3
IUPAC Name:
ethyl 2-cyclopentylidene-3-oxobutanoate
Constituent 2
Chemical structure
Reference substance name:
ethyl 2-(cyclopent-1-en-1-yl)-3-oxobutanoate
Molecular formula:
C11H16O3
IUPAC Name:
ethyl 2-(cyclopent-1-en-1-yl)-3-oxobutanoate
Specific details on test material used for the study:
Aspect : Colourless to pale yellow liquid
Lot No. : SC00018589
Purity : 93.8% (sum of the three peaks)
Expiration date : June 07, 2020


Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.

The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.
Duration of test (contact time):
61 d
Initial test substance concentration
Initial conc.:
30 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Key result
Parameter:
% degradation (O2 consumption)
Value:
66
Sampling time:
42 d
Parameter:
% degradation (O2 consumption)
Value:
75
Sampling time:
61 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
53
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable
Conclusions:
Ethyl Cyclopentylidene Acetoacetate did not significantly inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

Ethyl Cyclopentylidene Acetoacetate undergoes 53% biodegradation after 28 days (66% biodegradation after 42 days, 75% biodegradation after 61 days) in the test conditions.

Thus, Ethyl Cyclopentylidene Acetoacetate should be regarded as not readily biodegradable according to this test. However, based on these results Ethyl Cyclopentylidene Acetoacetate should be regarded as inherently and ultimately biodegradable.
Executive summary:

The Ready Biodegradability of Ethyl Cyclopentylidene Acetoacetate was determined by the Manometric Respirometry Test.

 

Ethyl Cyclopentylidene Acetoacetate did not significantly inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

 

Ethyl Cyclopentylidene Acetoacetate undergoes 53% biodegradation after 28 days (66% biodegradation after 42 days, 75% biodegradation after 61 days) in the test conditions.

 

Thus, Ethyl Cyclopentylidene Acetoacetate should be regarded as not readily biodegradableaccording to this test. However, based on these results Ethyl Cyclopentylidene Acetoacetate should be regarded as inherently and ultimately biodegradable.