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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Non-GLP near-guideline study, available as unpublished report, minor restrictions in design and reporting but the assessment is not possible since the volatilisation is expected to occur rapidly.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report date:
1976

Materials and methods

Principles of method if other than guideline:
Administration of the liquified test substance in right conjunctival sac of rabbit eye. One treated eye not washed; one treated eye washed with 0.9% saline for one minute.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chloro-1,1-difluoroethane
EC Number:
200-891-8
EC Name:
1-chloro-1,1-difluoroethane
Cas Number:
75-68-3
Molecular formula:
C2H3ClF2
IUPAC Name:
1-chloro-1,1-difluoroethane
Details on test material:
1-chloro, 1,1-difluoroethane
The purity of the test material was 99.9 %

Test animals / tissue source

Species:
other: Albino rabbit
Strain:
not specified

Test system

Vehicle:
not specified
Controls:
other: washed eye served as a negative control
Amount / concentration applied:
Amount applied: 0.1 ml
Duration of treatment / exposure:
0.33 minutes
Observation period (in vivo):
At 1 and 4 h , then at days one, two and five
Number of animals or in vitro replicates:
2

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
24 h
Score:
0
Max. score:
0
Irritation parameter:
iris score
Basis:
mean
Time point:
24 h
Score:
0
Max. score:
0
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
24 h
Score:
0
Max. score:
0
Irritation parameter:
chemosis score
Basis:
mean
Time point:
24 h
Score:
0
Max. score:
0
Irritant / corrosive response data:
There were no effects on the cornea and on the iris. On the conjunctiva , minimal swelling at 1h and slight transient discharge observed in unwashed (1h-1d) as well as in washed eye (1d). The substance produced pratically no ocular effect in either the unwashed and the washed rabbit's eye. This could be due to the fact that the compound is so low boiling (-9°C) that it did not stay in the eye (compound boiled out of eye immediately).

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The substance is practically not irritating to the eyes of rabbits.
Executive summary:

The instillation of 0.1 ml of liquified substance into rabbit eye led to minimal swelling of conjunctiva at 1h and slight transient discharge. There were no effects on the cornea and on the iris. However, as the compound has boiled out of the eye immediately, the relevance of this data to human risk assessment is very limited.