Barium bis[2-[(2-hydroxy-1-naphthyl)azo]benzoate]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

According to OECD and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Barium bis[2-chloro-5-[(2-hydroxy-1-naphthyl)azo]toluene-4-sulphonate]

EC Number:

225-935-3

EC Name:

Barium bis[2-chloro-5-[(2-hydroxy-1-naphthyl)azo]toluene-4-sulphonate]

Cas Number:

5160-02-1

Molecular formula:

C17H13ClN2O4S.1/2Ba

IUPAC Name:

barium bis{5-chloro-2-[(2-hydroxy-1-naphthyl)diazenyl]-4-methylbenzenesulfonate}

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

In order to determine the substance concentration of the test solution, samples were taken at study start (0 h) and study end (48 h); representative sample of total water amount 400 mL: 20 mL

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):
A saturated solution was tested in a limit test.
1 g of the test substance was weighed into a graduated 1000 mL flask, filled up with test water and stirred for about 24 h with a magnetic stirrer. The subspension was filtrated through a fluted filter. The filtrate, a clear light red solution, was used as test solution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna - Clone 5 (IRChA, France)
- Source: stock culture of the Department of Toxicology, Hoechst AG
- Method of breeding:
Culture: In 100 mL beakers filled with 70 mL culture medium, 1 daphnid per culture vessel up to an age of 42 days; the reproduction rate and the state of health were monitored daily apart from weekends.
Culture medium: demineralized water with a conductance of < 1 µS/cm according to Elendt
- Feeding during test: no feeding during the study

ACCLIMATION
- Acclimation period: not necessary; identical breeding and test conditions
- Type of food: monocellular green algae (Scenedesmus subspicatus)
- Feeding frequency: 3 times per week ad libitum

Reference:
Elendt, B. P. (1990): Selenium Deficiency in Crustacea, Protoplasma, 154, 25-30

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

80.1 Ca, 12.2 Mg, 144.9 HCO3 [mg/L]

Test temperature:

20.8 - 21.0 (concentration group)
20.2 - 21.0 (control)

pH:

7.6 - 8.3 (concentration group)
8.0 - 8.3 (control)

Dissolved oxygen:

8.3 - 8.7 (concentration group)
9.0 (control)

Nominal and measured concentrations:

measured concentrations:

control: < 0.1 mg/L (0 h and 48 h)
concentration group: 5.14 mg/L at 0 h; 2.39 mg/L at 48 h

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL glass beakers, height 110 mm, diameter 70 mm; 200 mL test water
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralized water with a conductance of < 1 µS/cm according to Elendt

OTHER TEST CONDITIONS
- Photoperiod: 12/12 h light/dark cycle
- Light intensity: illumination by fluorescent tubes diffuse light, illumination range max. 20 μE⋅m-2 ⋅s-1.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation at 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: Limit test with saturated solution (see above)
- Justification for using less concentrations than requested by guideline: limit test

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 3.8 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 3.8 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No immobilisation after 24 and 48 hours observed with the test substance (saturated solution) or the control.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

At the saturated solution (3.8 mg/L (mean)) of the test item no biologically significant effect was determined.

Executive summary:

In the acute immobilisation test with Daphnia magna the effect of the saturated solution (3.8 mg/L) of C.I. Pigment Red 53:1, was determined according to OECD 202 and Directive 79/831/EEC. The limit test was conducted under static conditions over 48 h. Water quality parameters pH-value and dissolved oxygen concentration, measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.

At the saturated solution of the test item, 3.8 mg/L (mean), no biologically significant effect was determined.

.