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Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1982
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
no
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Chlorobenzene
EC Number:
203-628-5
EC Name:
Chlorobenzene
Cas Number:
108-90-7
Molecular formula:
C6H5Cl
IUPAC Name:
chlorobenzene
Details on test material:
- Name of test material (as cited in study report): pure chlorobenzene
- Physical state: liquid
- Analytical purity: pure (no further data)

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: Hacking & Churchill ltd, Huntingdon, UK
- Weight at study initiation: approx 3.0 Kg
- Diet (e.g. ad libitum): Standard diet ("Ssniff K" from Versuchstierdiäten GmbH, Soest/Westfalen)
- Water (e.g. ad libitum): water was available ad libitum



ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-25°C
- Humidity (%): 40-60%
- Photoperiod (hrs dark / hrs light): 12 hours dark/12 hours light cycle


IN-LIFE DATES: November 1982

Test system

Vehicle:
not specified
Controls:
other: untreated eye used as control
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL
Duration of treatment / exposure:
24 hours
Observation period (in vivo):
21 days
Number of animals or in vitro replicates:
3
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): physiological solution of NaCl
- Time after start of exposure: 24 hours after the application of the test substance.


SCORING SYSTEM: The Draize scoring system was used to assess the irritatative/corrosive properties of the test substance.


TOOL USED TO ASSESS SCORE: fluorescein.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal #1
Time point:
24/48/72 h
Score:
0
Max. score:
4
Irritation parameter:
cornea opacity score
Basis:
animal #2
Time point:
24/48/72 h
Score:
0.3
Max. score:
4
Reversibility:
fully reversible within: 48 hrs
Irritation parameter:
cornea opacity score
Basis:
animal #3
Time point:
24/48/72 h
Score:
0
Max. score:
4
Irritation parameter:
iris score
Basis:
animal #1
Time point:
24/48/72 h
Score:
0
Max. score:
2
Irritation parameter:
iris score
Basis:
animal #2
Time point:
24/48/72 h
Score:
0
Max. score:
2
Irritation parameter:
iris score
Basis:
animal #3
Time point:
24/48/72 h
Score:
0
Max. score:
2
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
24/48/72 h
Score:
1
Max. score:
3
Reversibility:
fully reversible within: 7 days
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
24/48/72 h
Score:
1
Max. score:
3
Reversibility:
fully reversible within: 72 hrs
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
24/48/72 h
Score:
0.7
Max. score:
3
Reversibility:
fully reversible within: 72 hrs
Irritation parameter:
chemosis score
Basis:
animal #1
Time point:
24/48/72 h
Score:
0.3
Max. score:
4
Reversibility:
fully reversible within: 48 hrs
Irritation parameter:
chemosis score
Basis:
animal #2
Time point:
24/48/72 h
Score:
0.7
Max. score:
4
Reversibility:
fully reversible within: 72 hrs
Irritation parameter:
chemosis score
Basis:
animal #3
Time point:
24/48/72 h
Score:
0.3
Max. score:
4
Reversibility:
fully reversible within: 48 hrs
Other effects:
Compound related changes were not observed by evaluation according to Draize.

Any other information on results incl. tables

 





































































































































































 Readings Animal  Cornea Cornea Iris Conjunctiva  Conjunctiva  Conjunctiva
   Opacity Area Involved  Redness Chemosis Discharge
 24 h A 0 0 0 1 1 1
 24 h B 1 1 0 2 1 2
 24 h C 0 0 0 1 1 1
 48 hA 0 0 0 1 0 0
 48 h B 0 0 0 1 1 1
 48 h C 0 0 0 1 0 1
 72 hA 0 0 0 1 0 0
 72 h B 0 0 0 0 0 1
 72 h C 0 0 0 0 0 0
 Mean 24 -72h A 0  0 1 0.3 
 Mean 24 -72h B 0.3  0 1 0.7 
 Mean 24 -72h C 0  0 0.7 0.3 
 Total mean  0.1  0 0.9 0.4 
        

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: other: EU-GHS
Executive summary:

Suberg H., 1983.

The primary irritation/corrision effect of chlorobenzene on rabbits´eyes was tested according to "OECD Guideline for Testing of Chemicals No. 405" (without deviations). 3 New Zealand albino rabbits were treated 0.1 mL of the test substance in the conjunctival sac of one eye. The other eye was not treated and was used as control.

The ocular reactions were assessed 1, 24, 48, and 72 hours after application, and the evaluation was performed according to Draize.

The avarage score (24 to 72 hours) for irritation was calculated to be 0.1 for opacity, 0.9 for conjunctival redness , 0.4 for chemosis and 0 for iris. The ocular reactions were fully reversible in all animals within 7days after application.

According to EU-GHS regulations the pure chlorobenzene was classified as not irritant and not corrosive.