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Short-term toxicity to aquatic invertebrates

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Bobra et al., 1985
Although no standard guideline was followed, the conditions of this test yield similar results to the other studies with daphnids. In this test 50% immobilisation with Daphnia magna was obtained after 48 h at 0.59 mg/L, based on nominal concentrations.
As daphnids are the most sensitive aquatic organisms, the classification is based on the results obtained in Daphnia magna. Many studies on short term toxicity to aquatic invertebrates are summarised in the BUA report (BUA 1990). In table 55 in the BUA report either immobilisation or mortality data for Daphnia magna are reported from studies published between 1980 and 1987. Ten LC50/EC50 values were obtained in closed systems. One study resulted in an EC50 of 0.59 mg/L, nine studies showed LC50/EC50 values between 4.3 and 140 mg/L. The only test, in which the test concentrations were analytically verified, yielded an EC50 of 26 mg/L. Due to "weight of evidence" it is assumed that current classification with respect to the toxicity to aquatic organisms is R 51. This is in agreement to the discussions on the environmental effects classification in the Commission Working Group 3-5 April 1995 (European Commission (1995). Summary record ¿ Commission Working Group on the Classification and Labelling of dangerous substances: Environmental Effects. ECBI/39/95 ¿ Rev. 1).

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