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Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1985-03-11 to 1985-05-07
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1985
Report date:
1985

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Trioctylphosphine
EC Number:
225-234-2
EC Name:
Trioctylphosphine
Cas Number:
4731-53-7
Molecular formula:
C24H51P
IUPAC Name:
trioctylphosphane
Test material form:
liquid

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: Hazleton-Dutchland, Inc. Denver, Pennsyvania
- Age at study initiation: Young adults
- Housing: Individually housed
- Cages: Suspended, stainless steel
- Diet (e.g. ad libitum): Lab Rabbit Chow HF (Purina #5326)
- Water (e.g. ad libitum): Automatic watering system Municipal water supply (Elyzabethtown water Co.)
- Acclimation period: 15 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 15.6 to 21.1 °C (60 - 70 °F)
- Humidity (%):30 - 70 %
- Photoperiod (hrs dark / hrs light): 12h / 12h

IN-LIFE DATES: From: To:1985-05-26 to 1985-05-29

Test system

Vehicle:
unchanged (no vehicle)
Controls:
yes, concurrent negative control
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit):0.1 mL
Observation period (in vivo):
3 days
Number of animals or in vitro replicates:
6 (2 males and 4 females)
Details on study design:
SCORING SYSTEM: Draize Scale

TOOL USED TO ASSESS SCORE: fluorescein dye was used to confirm presence of absence of corneal ulceration.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
maximum mean total score (MMTS)
Basis:
animal #1
Time point:
24 h
Score:
10
Max. score:
110
Reversibility:
not specified
Irritation parameter:
maximum mean total score (MMTS)
Basis:
animal #2
Time point:
24 h
Score:
10
Max. score:
110
Reversibility:
not specified
Irritation parameter:
maximum mean total score (MMTS)
Basis:
animal #3
Time point:
24 h
Score:
8
Max. score:
110
Reversibility:
not specified
Irritation parameter:
maximum mean total score (MMTS)
Basis:
animal #4
Time point:
24 h
Score:
10
Max. score:
110
Reversibility:
not specified
Irritation parameter:
maximum mean total score (MMTS)
Basis:
animal #5
Time point:
24 h
Score:
12
Max. score:
110
Reversibility:
not specified
Irritation parameter:
maximum mean total score (MMTS)
Basis:
animal #6
Time point:
24 h
Score:
12
Max. score:
110
Reversibility:
not specified

Any other information on results incl. tables

Summary of responses and maximum Draize Scores

 

Animal number

Cornea

Iris

Conjunctivae

Score

First time obtained

Time clearb

 

Opacity

Ulceration

 

Redness

Chemosis

Necrosis or Ulceration

 

 

 

6422 F

-

-

-

-

+

-

10

24h

C

6423 M

-

-

-

-

+

-

10

24h

C

6424 F

-

-

-

-

-

-

8

24h

C

6426 F

-

-

-

-

+

-

10

24h

C

6427 M

-

-

-

+

+

-

12

24h

C

6406 F

-

-

-

+

+

-

12

24h

C

b : time clear is time that eye was free of all irritation

C: Not clear at termination of study (Day 3)

M: male

F: female

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritating to eyes) based on GHS criteria
Conclusions:
The substance produced generally mild irritation which consisted primarilly of conjunctival redness, chemosis and discharge, with the most severe effects occuring at 24h. In five o the six animals, scores fo conjunctival chemosis and/or redness were considered positive irritant response. No corneal ulceration or opacity or iridial damage were seen. Periorbital irritaion (erythema, chemosis, alopecia, necrosis and:or desquamation) was evident in all six animals. Irritation persisted through study termination (72h), although some recovery from the 24-hour observations was apparent.
Executive summary:

In an in vivo eye irritation study, 0.1 mL of trioctylphosphine was applied into eye of rabbit.

Observations at 24h, 48h and 72h after application showed effects on Conjuntivae and Cornea.

Taking into accoun t thereulsts, the substance was considered to be irritating to the eye.

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