4-[(4-ethoxyphenyl)amino]-N,N-dimethyl-3-nitrobenzenesulphonamide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Remarks:

BOD, COD

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

From September 15, 1993 to September 22, 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

The test was conducted on a similar substance. The complete justification for the read across is attached at section 13.

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Version / remarks:

DEV DIN 38'409 - H 41-1 (1980) corresponding to 92 / 96 / EEC C.6.

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

Principles of method if other than guideline:

Internal method: SAV: 02 000 02

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

not specified

Inoculum or test system:

not specified

Duration of test (contact time):

ca. 2 h

Reference substance:

other: Potassium hydrogen phthalate solution

Remarks on result:

not determinable

Parameter:

COD

Value:

1 412 mg O2/g test mat.

Results with reference substance:

The COD of Potassium hydrogen phthalate is (Criteria: 200 ± 8 mg O2/I) : 201 mg O2/I

COD = 1412 mg O2/g

Validity criteria fulfilled:

not applicable

Interpretation of results:

other: not applicable

Conclusions:

The COD of test item is 1412 mg O2/g
The COD of the reference substance is 201 mg O2/I

Executive summary:

The test was carried out according to DEV DIN 38'409 - H 41-1 (1980) corresponding to 92/96/EEC C.6. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.

A predeterminated amount of the test substance in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate.

The test substance was tested with a sample weight of 7.39 + 7.11 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I.

The COD of test item is 1412 mg O2/g

The COD of the reference substance is 201 mg O2/I

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

From November 17, 1993 to December 01, 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The test was conducted on a similar substance. The complete justification for the read across is attached at section 13.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

yes

Remarks:

(instead of 28 d the test has been finished after 14 d since no degradation activity could be observed)

Qualifier:

according to guideline

Guideline:

other: EEC-Directive, 79/831, Annex V, C.7. ABIOTIC DEGRADATION HYDROLYSIS AS A FUNCTION OF PH

Principles of method if other than guideline:

Internal method: SAV : 06 000 02

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

Strain / Inoculation: Mixture of polyvalent bacteria collected in the aeration tank of a domestic sewage treatment plant, ARA Basel Communal
Bacteria concentration: 30 mg/L

Duration of test (contact time):

ca. 14 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Water/Nutrient: According to the specification of the test method
Temperature: 20 ± 2 °C
Lighting: None
Estimation Technique: Voith Sapromat; O2-Consumption automatically measured

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (O2 consumption)

Value:

ca. 0

Sampling time:

14 d

Details on results:

The biodegradation of test substance (corrected by the inoculum control) expressed as the % of BOD versus ThOD was 0 % (14 d).

Results with reference substance:

The biodegradation (corrected by the inoculum control) expressed as the % of BOD versus ThOD was 91 % (14 d).

Validity CRITERIA:

The degradation after 14 days of the reference substance (Natriumbenzoat) has to be higher than 65 %.

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Under the study conditions, the test substance was not readily biodegradable.

Executive summary:

The ready biodegradability of the test substance was determined in a 14 d MITI test (I) according to OECD Guideline 301C (1981) and the EU Method C.7. Both the test substance and reference substance sodium benzoate were tested at a concentration of 100 mg/l. Biodegradation was equivalent to 0 and 91 %, respectively. Under the study conditions, the test substance was not readily biodegradable.

Description of key information

Not readily biodegradable