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EC number: 221-717-7 | CAS number: 3209-22-1
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- The bioconcentration factor of a series of nitrobenzenes derivatives was determined for the guppy.
- GLP compliance:
- not specified
- Radiolabelling:
- no
- Details on sampling:
- - Sampling intervals/frequency for test organisms: After 1 and 2 days of exposure two guppies were taken for analysis; at the end of day three all remaining fish were taken.
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Common name: guppy
- female guppies were used
- Age at study initiation: 5 to 8 month old
- Weight at study initiation: wet weight varied from 60 to 450 mg at the end of the experiment; mean fat content was 8 +/-2 %
- Food type: commercial fishfood
- Frequency: daily
ACCLIMATION
- Acclimation period: at least 12 days - Route of exposure:
- aqueous
- Test type:
- semi-static
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 3 d
- Hardness:
- 25 mg/l CaCO3
- Details on test conditions:
- TEST MEDIUM / WATER PARAMETERS
- water for all experiments: standard water (SW) prepared according to Alabaster and Abram (1964), with corresponds to very soft tap water (hardness: 25 mg/l as CaCO3)
TEST SYSTEM
- Test vessel: glas jars of 1.5 l containing 1 l of solution
- Renewal rate of test solution: daily
- No. of organisms per vessel: 9 female guppies
TEST MEDIUM / WATER PARAMETERS
- water for all experiments: standard water (SW) prepared according to Alabaster and Abram (1964), with corresponds to very soft tap water (hardness: 25 mg/l as CaCO3) - Reference substance (positive control):
- not specified
- Key result
- Type:
- BCF
- Value:
- 1 027 dimensionless
- Basis:
- whole body w.w.
- Remarks on result:
- other: Conc.in environment / dose:840 µg/l
- Type:
- BCF
- Value:
- 82 dimensionless
- Basis:
- total lipid content
- Remarks on result:
- other: average lipid content: 8 +/- 2 %
- Remarks:
- Conc.in environment / dose:840 mg/l
- Conclusions:
- The bioaccumulation factor (BCF) was determined in an experimental study. Guppies (poecilia reticulata) were exposed to 1,2-dichloro-3-nitrobenzene for 3 days under semi-static conditions in freshwater. A BCF of 1027 +/-95 (N=7); whole body w.w. was determined.
- Executive summary:
Deneer, 1987
The bioaccumulation factor (BCF) was determined in an experimental study. Guppies (poecilia reticulata) were exposed to 1,2-dichloro-3-nitrobenzene for 3 days under semi-static conditions in freshwater.
BCF: 1027 +/-95 (N=7); whole body w.w.
log BCF: 3.01 +/- 0.04; whole body w.w.
BCF: 82 +/- 20; total lipid content
In same preliminary experiments it was established that the uptake of the chemicals proceeded very fast, thus enabling the use of this short-term assay.
Reference
results:
BCF: 1027 +/-95 (N=7); whole body w.w.
log BCF: 3.01 +/- 0.04; whole body w.w.
BCF: 82 +/- 20; total lipid content
In same preliminary experiments it was established that the uptake of the chemicals proceeded very fast, thus enabling the use of this short-term assay.
Description of key information
Deneer, 1987
The bioaccumulation factor (BCF) was determined in an experimental study. Guppies (poecilia reticulata) were exposed to 1,2-dichloro-3-nitrobenzene for 3 days under semi-static conditions in freshwater. The following experimental values were obtained:
BCF: 1027 +/-95 (N=7); whole body w.w.
log BCF: 3.01 +/- 0.04; whole body w.w.
BCF: 82 +/- 20; total lipid content
In same preliminary experiments it was established that the uptake of the chemicals proceeded very fast, thus enabling the use of this short-term assay.
The BCF value obtained from this study (1027, whole body w.w.) is assumed as most conservative value.
Key value for chemical safety assessment
- BCF (aquatic species):
- 1 027
Additional information
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