Methyl anthranilate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

The objective of the study was determine percent biodegradation of test chemical in water for 20 days.

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

other: Micro-organism

Duration of test (contact time):

20 d

Initial conc.:

50 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

test mat. analysis

Key result

Parameter:

% degradation (O2 consumption)

Value:

100

Sampling time:

20 d

Remarks on result:

other: other details not known

Details on results:

Study was conducted to determine percent degradation of test chemical in water. Under conditions of optimal bacterial growth (warmth and darkness) biodegradation of test chemical was determined to be 100% in 20 days.

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

Study was conducted to determine percent degradation of test chemical in water. Under conditions of optimal bacterial growth (warmth and darkness), biodegradation of test chemical was determined to be 100% in 20 days.

Executive summary:

The principle of the study was to determine biodegradation rate of test chemical in water. Study was carried out for 20 days under the condition of optimal bacterial growth. Test chemical was purchased from Fluka Chemical Company. The initial concentration of test chemical used in the study was 50 mg/L. Test chemical concentration was determined by High performance liquid chromatography (HPLC). The HPLC system consisted of a Rainin HPXL solvent delivery system, Dynamax A1-2 autosampler equipped with a 2 0 4 fixed sample loop and a Dynamax UV-M ultraviolet detector. Quantitative analysis of test chemical was done by using Hewlett Packard 3390A recording integrator. Brucker MSL-400 (400 MHz) NMR spectrometer, Finnegan GC/MS spectrometer equipped with a Data General Nova computer system, a Shimadzu IR-435 infrared spectrophotometer and a Gilford 2600 ultraviolet spectrophotometer were used for the spectral analysis. Approximately 50 mg liter of two solution of test chemical were prepared in dechlorinated, charcoal-filtered Vessels which were stored open to natural inoculation in a water bath maintained at 13-15°C for seven days prior to initiation of the test. One tank received 1 g of sodium azide, a metabolic poison after seven days resulting in a final concentration of 0.1 g liter. There is no further treatment given to control tank. 100 ml of samples were drawn, sealed (to prevent further inoculation) and stored at 23°C in sterilized, sealed amber vials. Vials were stored under dark or light: dark (12: 12 h) conditions. Aliquots from all treatments were analyzed for test chemical content at intervals of 0, 0.2, 1, 2, 5, 6, 7, 8, 9 and 20 days post-treatment. Under conditions of optimal bacterial growth (warmth and darkness), biodegradation of test chemical was determined to be 100% in 20 days. Based on the test chemical analysis, test chemical determined to be readily biodegradable in water.

Description of key information

The principle of the study was to determine biodegradation rate of test chemical in water. Study was carried out for 20 days under the condition of optimal bacterial growth. Test chemical was purchased from Fluka Chemical Company. The initial concentration of test chemical used in the study was 50 mg/L. Test chemical concentration was determined by High performance liquid chromatography (HPLC). The HPLC system consisted of a Rainin HPXL solvent delivery system, Dynamax A1-2 autosampler equipped with a 2 0 4 fixed sample loop and a Dynamax UV-M ultraviolet detector. Quantitative analysis of test chemical was done by using Hewlett Packard 3390A recording integrator. Brucker MSL-400 (400 MHz) NMR spectrometer, Finnegan GC/MS spectrometer equipped with a Data General Nova computer system, a Shimadzu IR-435 infrared spectrophotometer and a Gilford 2600 ultraviolet spectrophotometer were used for the spectral analysis. Approximately 50 mg liter of two solution of test chemical were prepared in dechlorinated, charcoal-filtered Vessels which were stored open to natural inoculation in a water bath maintained at 13-15°C for seven days prior to initiation of the test. One tank received 1 g of sodium azide, a metabolic poison after seven days resulting in a final concentration of 0.1 g liter. There is no further treatment given to control tank. 100 ml of samples were drawn, sealed (to prevent further inoculation) and stored at 23°C in sterilized, sealed amber vials. Vials were stored under dark or light: dark (12: 12 h) conditions. Aliquots from all treatments were analyzed for test chemical content at intervals of 0, 0.2, 1, 2, 5, 6, 7, 8, 9 and 20 days post-treatment. Under conditions of optimal bacterial growth (warmth and darkness), biodegradation of test chemical was determined to be 100% in 20 days. Based on the test chemical analysis, test chemical determined to be readily biodegradable in water.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater

Additional information

Following different studies includes experimental study for the test chemical and for read-across analogues which is extracted by using mechanistic approach and functionally and structurally similar to the test chemical to observe the biodegradation rate of test chemical in water.

 

The principle of the first study was to determine biodegradation rate of test chemical in water. Study was carried out for 20 days under the condition of optimal bacterial growth. Test chemical was purchased from Fluka Chemical Company. The initial concentration of test chemical used in the study was 50 mg/L. Test chemical concentration was determined by High performance liquid chromatography (HPLC). The HPLC system consisted of a Rainin HPXL solvent delivery system, Dynamax A1-2 autosampler equipped with a 2 0 4 fixed sample loop and a Dynamax UV-M ultraviolet detector. Quantitative analysis of test chemical was done by using Hewlett Packard 3390A recording integrator. Brucker MSL-400 (400 MHz) NMR spectrometer, Finnegan GC/MS spectrometer equipped with a Data General Nova computer system, a Shimadzu IR-435 infrared spectrophotometer and a Gilford 2600 ultraviolet spectrophotometer were used for the spectral analysis. Approximately 50 mg liter of two solution of test chemical were prepared in dechlorinated, charcoal-filtered Vessels which were stored open to natural inoculation in a water bath maintained at 13-15°C for seven days prior to initiation of the test. One tank received 1 g of sodium azide, a metabolic poison after seven days resulting in a final concentration of 0.1 g liter. There is no further treatment given to control tank. 100 ml of samples were drawn, sealed (to prevent further inoculation) and stored at 23°C in sterilized, sealed amber vials. Vials were stored under dark or light: dark (12: 12 h) conditions. Aliquots from all treatments were analyzed for test chemical content at intervals of 0, 0.2, 1, 2, 5, 6, 7, 8, 9 and 20 days post-treatment. Under conditions of optimal bacterial growth (warmth and darkness), biodegradation of test chemical was determined to be 100% in 20 days. Based on the test chemical analysis, test chemical determined to be readily biodegradable in water.

 

Supporting study was carried out to determine biodegradation of test chemical in water. Test was conducted in accordance with EPA OTS 796.3260 (Ready Biodegradability: Modified Sturm Test) for 29 days. Activated sewage sludge was used as test inoculums for the study. The initial concentration of test chemical used in the study was 28 mg/L. Based on the carbon dioxide evolution, the percent degradation of test chemical was determined to be 62% in 29 days. Thus on the basis of determined percent degradation value, test chemical is considered to be readily biodegradable in water.

 

The next biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test chemical. Activated sludge was used as test inoculums for the study. Concentration of inoculum i.e., sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test chemical was determined to be 85, 99 and 100% degradation by O2 consumption (BOD (NH3)), TOC removal and test material analysis by HPLC parameter in 14 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in water.

 

On the basis of above determined values, test chemical is considered to be readily biodegradable in water.