3-methylbut-2-en-1-ol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

reduced post observation time  (7 days instead of 14)

Principles of method if other than guideline:

In principle, the methods described in the OECD Guideline 401 were used. Several groups of 10 rats per sex and dose were treated simultaneously by gavage with preparations of the test substance in suitable vehicle.

GLP compliance:

no

Remarks:

GLP was not compulsory at the time the study was conducted.

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-2-en-1-ol

EC Number:

209-141-4

EC Name:

3-methylbut-2-en-1-ol

Cas Number:

556-82-1

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-2-en-1-ol

Details on test material:

Name of test substance: 3-Methylbut-2-en-1-ol (Prenol)
Purity: 95 %
Impurities: no further data on impurities given

Test animals

Species:

rat

Strain:

other: Gassner

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Average weight at study initiation: 160-260 g

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: water; emulsion in water containing traganth

Details on oral exposure:

- Concentration in vehicle: The doses were administered at concentrations of 2% (0.2 ml/kg bw), 20% (1.6, 2.0 and 2.5 ml/kg bw), and 30% (3.2 and 6.4 ml/kg bw) aqueous emulsions, respectively.

Doses:

172, 1376, 1720, 2150, 2752 and 5504 mg/kg bw;
the original values were reported as 0.2, 1.6, 2.0, 2.5, 3.2 and 6.4 ml/kg bw. The mg/kg bw doses were based on a density of 0.86 g/ml at 20 °C.

No. of animals per sex per dose:

10 animals per sex per dose.

Control animals:

no

Details on study design:

Animals were inspected for signs of pharmacologic or toxicologic effects during a 7 days post observation period.
Body weight was measured before dosing. 
At the end of the observation period, survivors were sacrificed and necropsied as were animals that died.
The LD50 value was estimated (calculation method not mentioned in the raw data).

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 591 mg/kg bw

Remarks on result:

other: LD50 value was reported to be ca. 1.85 ml/kg bw

Mortality:

No deaths occurred at the lower doses up to 1.6 ml/kg bw. All animals died at 2.0 ml/kg bw and above.

Time of death and number of deaths at each dose:
- 6.4 ml/kg bw: 10/10 males and 10/10 females died within 24 hrs
- 3.2 ml/kg bw: 10/10 males and 10/10 females died within 24 hrs
- 2.5 ml/kg bw: 9/10 males and 10/10 females died within 24 hrs
- 2.0 ml/kg bw: 7/10 males and 10/10 females died within 24 hrs
- 1.6 ml/kg bw: 0/10 males and 0/10 females died within 7 days
- 0.2 ml/kg bw: 0/10 males and 0/10 females died within 7 days

From these data a LD50 value was estimated to be 1591  mg/kg bw after 7 days. The original LD50 value was reported to be ca. 1.85 ml/kg bw.

Clinical signs:

other: At 6.4 to 2.0 ml/kg bw:  Immediately after application, staggering, abdominal and lateral position, partly dorsal position, apathy, laboured and irregular breathing, secretion out of eyes and mouth, reddened eyes and ears. In the post observation days, ca

Gross pathology:

In 2 animals that died after application of 2.5 ml/kg bw, stomach dilated and filled with liquid, all other animals without findings in the inner organs.