Registration Dossier
Registration Dossier
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EC number: 253-197-2 | CAS number: 36768-62-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Triacetonediamine
- IUPAC Name:
- Triacetonediamine
- Reference substance name:
- 2,2,6,6-tetramethyl-4-piperidylamine
- EC Number:
- 253-197-2
- EC Name:
- 2,2,6,6-tetramethyl-4-piperidylamine
- Cas Number:
- 36768-62-4
- Molecular formula:
- C9H20N2
- IUPAC Name:
- 2,2,6,6-tetramethylpiperidin-4-amine
Constituent 1
Constituent 2
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- A fresh sample of activated sludge from a municipal sewage treatment plant in Moers (Germany) was collected and aerated until usage. The inoculum was not fed during the aeration period of 2 days. After determination of the suspended solids (dry weight) a sample containing approx. 1875 mg suspended solids was filtered, the sludge was washed twice with mineral medium. Afterwards it was suspended in 250 ml mineral medium and stirred unitl it was homogenous. 4 ml of this suspension were used to inoculate 1 litre if mineral medium. This amount of inoculum corresponded to 30 mg/l suspended solids.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- ThOD/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 0 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- -3
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- -2.6
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- -3
- Sampling time:
- 21 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- -4.6
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 105% degradation within 28 d, 90% after 14 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Under present conditions tested, test substance is not readily biodegradable.
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