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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999-11-15 to 1999-12-10
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
m-acetylphenyl acetate
EC Number:
219-524-8
EC Name:
m-acetylphenyl acetate
Cas Number:
2454-35-5
Molecular formula:
C10H10O3
IUPAC Name:
3-acetylphenyl acetate
Test material form:
solid

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Source: supplied 1997 by the Umweltbundesamt, Institut für Wasser-, Boden- und
Lufhygiene, Berlin, Germany
- Age of parental stock (mean and range, SD): 8 - 23.5 hours

ACCLIMATION
- Acclimation period: for 8 hours under test conditions

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.5 mmol/L (=250.0 mg/L) as CaCO3
Test temperature:
21°C
pH:
pH 7.6 to 7.8
Dissolved oxygen:
at least 7.5 mg/L or higher
Nominal and measured concentrations:
- Nominal concentration: 4.6, 10, 21, 46, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL glass beaker
- No. of vessels per concentration (replicates): 20 Daphnia, divided into two groups of ten animals, each group in 50 mL test medium
- No. of vessels per control (replicates): 20 Daphnia, divided into two groups of ten animals, each group in 50 mL test medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water prepared according to guideline

OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark

RANGE-FINDING STUDY
- Test concentrations: not above 100 mg substance/L

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
90.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
21 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
21 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
46 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Results after 24 h and 48 h

Nominal concentration [mg/L]

Results after 24 h and 48 h

Immobile daphnids

Oxygen content [mg/L) after 48 h

pH after 48 h

Absolute

%

24 h

48 h

24 h

48 h

0 (control)

0

0

0

0

8.1

7.8

4.6

0

0

0

0

8.1

7.8

10

0

0

0

0

8.0

7.8

21

0

0

0

0

8.0

7.8

46

0

2

0

10

7.8

7.7

100

6

15

30

75

7.7

7.6

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48-h EC50 was determined to be 90.9 mg/L.
Executive summary:

The 48 hour EC50 was calculated to be 90.9 mg test substance/L with 95% confidence limits from 51.7 to 159.6 mg/L, according to OECD TG 202 (1984).

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