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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experimental Phase: 17 October 2018 to 31 October 2018. Report Issue:
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
other: HPLC column containing lipophilic and polar moieties.
Details on study design: HPLC method:
HPLC System : Agilent Technologies 1200, incorporating workstation and autosampler
Detector type : variable wavelength (VW)
Column : Waters XSelect CN 5μm (150 x 4.6 mm id)
Column temperature : 30 °C
pH of mobile phase : 6.5
Flow-rate : 1.0 mL/min
Injection volume : 10 μL
VW detector wavelength : 210 nm

PREPARATION OF SAMPLE SOLUTION
Test item (0.0225 g) was diluted to 100 mL with methanol, to give a concentration of 225 mg/L.

MOBILE PHASE
Type: Methanol: purified water (55:45 v/v)

DETEMINATION OF DEAD TIME
Method: The dead time was determined by measuring the retention time of a formamide (purity* 99.6%) of a 671 mg/L solution in mobile phase.

REFERENCE SUBSTANCES
Identity: Acetanilide, Phenol, Atrazine, Isoproturon, Triadimenol, Linuron, Naphthalene, Fenthion, α-Endosulfan, Diclofop-methyl, Phenanthrene, DDT.
Key result
Type:
Koc
Value:
> 430 000 dimensionless
Temp.:
30 °C
Key result
Type:
log Koc
Value:
> 5.53 dimensionless
Temp.:
30 °C

The retention times, capacity factor and log10 Koc value determined for the sample are shown in the following table:

 Injection

 Retention Time

(min)

 Capcity Factor

(K')

Log10 K'   Log10 Koc
 1  ≥ 31.696  > 10.324 > 1.014  >  5.63
 2  ≥ 31.696  > 10.324 > 1.014 >  5.63

Mean log10 Koc: >5.63

Adsorption coefficient: >4.3 x 105

Validity criteria fulfilled:
yes
Conclusions:
The adsorption coefficient of the test item has been determined to be greater than 4.3 x 10^5, log10 Koc >5.63. The results classify the test item as being immobile.
Executive summary:

Introduction

The Adsorption coefficient of the test item was determined using a HPLC screening method designed to be compatible with the following guidelines:

  1. Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008

  2. Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001.

Conclusion

The adsorption coefficient of the test item has been determined to be greater than 4.3 x 105, log10 Koc >5.63. The results classify the test item as being immobile.

Description of key information

Absorption Coefficient of the test substance by HPLC screening method.

Key value for chemical safety assessment

Koc at 20 °C:
430 000

Additional information