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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
genetic toxicity in vivo
Remarks:
Type of genotoxicity: other: review paper covering many studies
Type of information:
other: published data
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.

Data source

Reference
Reference Type:
review article or handbook
Title:
Carbon disulfide. Priority substances list assessment report.
Author:
Canadian Environmental Protection Act
Year:
2000
Bibliographic source:
Minister of Public Works and Government Services: Ottawa, Canada

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Review of a large series of publications on studies in which a wide array of methods was applied.
GLP compliance:
not specified
Type of assay:
other: review paper covering many studies

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: Not applicable: review paper.
Details on test material:
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.
- Name of test material (as cited in study report): carbon disulphide
- Molecular formula (if other than submission substance): CS2
- Physical state: liquid
- Analytical purity: >99.8%
- Impurities (identity and concentrations): iron, <1 mg/kg; sulfide <3 mg/kg
- Purity test date: 03.07.1992
- batch No.: 0609206042530
- Storage condition of test material: kept cool in the original container
- Density: 1.264 g/cm2

Test animals

Species:
other: A series of studies with various animal species was reviewed.
Strain:
other: A series of studies with various animal species was reviewed.
Sex:
male/female
Details on test animals and environmental conditions:
Not applicable: review paper.

Administration / exposure

Route of administration:
other: Invasive (injection) and non-invasive (inhalation, oral, dermal) routes were employed in the different studies reviewed.
Vehicle:
Not applicable: review paper.
Details on exposure:
Not applicable: review paper.
Duration of treatment / exposure:
Not applicable: review paper.
Frequency of treatment:
Not applicable: review paper.

Results and discussion

Applicant's summary and conclusion

Conclusions:
Interpretation of results : negative
In the majority of the in vivo tests assessing CS2 mutagenic potential, a negative result was obtained, except for one case. The validity of the studies is uncertain due to technical issues (e.g. invalid controls).
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.
Executive summary:

In male and female rats inhaling 63 or 125 mg carbon disulfide/m3, 7 hours per day for 1 or 5 days, there was no significant increase in the frequency of chromosomal aberrations in bone marrow cells (Belisles et al., 1980). In another study (Vasil’eva, 1982), oral exposure to carbon disulfide gave a mutagenic response, manifested as chromosomal aberrations and polyploid cells in the bone marrow of female rats and in rat embryos exposed on days 10–13 of gestation. According to the reviewer,'it is difficult to assess the validity of these findings, as the reporting was brief e.g., the statistical significance was often not indicated and the effective dose was not reported, except to indicate that it was one-tenth of the LD50 '. In the investigation of Belisles et al. (1980), male rats were exposed to 63–125 mg/m3 of CS2, 7 h/d for 5 d; no significant increase in dominant lethal mutations was observed, nor was there a dose related increase in sperm abnormalities in rats or mice exposed according to the same protocol. However, lack of an effect on sperm abnormalities in positive control rats suggests that there was a problem with the test methods in this study.