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Endpoint:
activated sludge respiration inhibition testing
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to
Guideline:
other: A-Z test, Deutsches Einheidsverfahren DEV-L 9
Principles of method if other than guideline:
Measurement of oxygen consumption of activated sludge in a closed system to prevent evaporation of CS2.
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
Mixed bacterial population (activated sludge).
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Hardness:
NR
Test temperature:
20 ± 1 °C
pH:
8.7 ± 0.2
Dissolved oxygen:
Not reported
Salinity:
Not reported
Details on test conditions:
not available
Reference substance (positive control):
not required
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
13 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
4.6 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
34.89 mg/L
Nominal / measured:
nominal
Conc. based on:
other: S-allyl O-pentyl dithiocarbonate
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: On a molecular weight scaled basis, the EC50 would be 34.89 mg/l. (13 x 204.35) /76.13 =34.89 mg/l
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
12.35 mg/L
Nominal / measured:
nominal
Conc. based on:
other: S-allyl O-pentyl dithiocarbonate
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: On a molecular weight scaled basis, the EC10 would be 12.35 mg/l. (4.6x 204.35) /76.13 =12.35 mg/l
Details on results:
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.
On a molecular weight scaled basis, the EC50 would be 34.89 mg/l. (13 x 204.35) /76.13 =34.89 mg/l
On a molecular weight scaled basis, the EC10 would be 12.35 mg/l. (4.6x 204.35) /76.13 =12.35 mg/l

.

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for inhibition of oxygen consumption in activated sludge by CS2 is 13 mg/L.
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.
On a molecular weight scaled basis, the EC50 would be 34.89 mg/l. (13 x 204.35) /76.13 =34.89 mg/l
On a molecular weight scaled basis, the EC10 would be 12.35 mg/l. (4.6x 204.35) /76.13 =12.35 mg/l


Executive summary:

The EC50 for inhibition of oxygen consumption in activated sludge by CS2 is 13 mg/L.

Carbon disulphide (CAS number 75–15–0)  is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.

On a molecular weight scaled basis, the EC50 would be 34.89 mg/l.  (13 x 204.35) /76.13 =34.89 mg/l

On a molecular weight scaled basis, the EC10 would be 12.35 mg/l.  (4.6x 204.35) /76.13 =12.35 mg/l

Endpoint:
activated sludge nitrification inhibition testing
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.
Qualifier:
no guideline available
Principles of method if other than guideline:
The Microtox test for reduction of luminescence in Photobacterium phosphoreum was used. The test was carried out using the procedure described in the manual of the Beckman 2055 apparatus (1982).
GLP compliance:
no
Analytical monitoring:
not required
Vehicle:
no
Test organisms (species):
Photobacterium phosphoreum
Details on inoculum:
marine bacterium
Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
15 min
Hardness:
90 mg CaCO3/L
Test temperature:
20 ± 1 °C
pH:
8.7 ± 0.2
Dissolved oxygen:
Not reported
Salinity:
Not reported
Nominal and measured concentrations:
The test was carried out in sealed flasks to prevent evaporation of CS2.
The medium contained major salts, urea, Ca-glycerophosphate, dextrose and trace elements. The complete composition of the test medium is given in Van Leeuwen and Maas, Environ. Pollution A37: 105-115 (1985).
Details on test conditions:
The test was carried out in sealed flasks to prevent evaporation of CS2.
Reference substance (positive control):
no
Duration:
15 min
Dose descriptor:
EC50
Effect conc.:
341 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: inhibition of bacterial luminescence
Remarks on result:
other: 95% C.I.: 260-448 mg/L
Duration:
15 min
Dose descriptor:
EC50
Effect conc.:
915.32 mg/L
Nominal / measured:
nominal
Conc. based on:
other: S-allyl O-pentyl dithiocarbonate
Basis for effect:
other: inhibition of bacterial luminescence
Remarks on result:
other: On a molecular weight scaled basis, the EC50 would be 915.32 mg/l. (341 x 204.35) /76.13 =915.32 mg/l
Details on results:
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.
On a molecular weight scaled basis, the EC50 would be 915.32 mg/l. (341 x 204.35) /76.13 =915.32 mg/l

This study reports a 15-min EC50 of 341 mg/L (carbon disulphide (CS2) for the marine bacterium Photobacterium phosphoreum based on the results of a Microtox test.

Validity criteria fulfilled:
yes
Conclusions:
The 15-min EC50 of CS2 for Photobacterium posphoreum luminescence was 341 mg/L.
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.
On a molecular weight scaled basis, the EC50 would be 915.32 mg/l. (341 x 204.35) /76.13 =915.32 mg/l
Executive summary:

The 15-min EC50 of CS2 for Photobacterium posphoreum luminescence was 341 mg/L.

Carbon disulphide (CAS number 75–15–0)  is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.

On a molecular weight scaled basis, the EC50 would be 915.32 mg/l.  (341 x 204.35) /76.13 =915.32 mg/l

Endpoint:
activated sludge nitrification inhibition testing
Type of information:
other: published data
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
The S-allyl O-pentyl dithiocarbonate (CAS No. 2956-12-9), the subject of this dossier) is expected to exhibit very similar toxicity to Potassium ethyl xanthate (CAS No. 140-89-6), which is also xanthate compound. Comparable metabolism would occur.
Qualifier:
equivalent or similar to
Guideline:
ISO 9509 (Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms)
Principles of method if other than guideline:
The influence of 28 nitrification inhibitors on denitrification of nitrate in soil was studied by determining the effects of different amounts of each inhibitor on the amounts of nitrate lost and the amounts of nitrite, N2O and N2 produced when soil samples were incubated anaerobically after treatment with nitrate or with nitrate and mannitol.
GLP compliance:
not specified
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Nitrosomonas sp.
Details on inoculum:
Nitrosomonas
Scientific classification
Domain: Bacteria
Phylum: Proteobacteria
Class: Beta Proteobacteria
Order: Nitrosomonadales
Family: Nitrosomonadaceae
Genus: Nitrosomonas
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Hardness:
NR
Test temperature:
20 to 30°C
pH:
6.0-9.0
Dissolved oxygen:
NR
Salinity:
NR
Nominal and measured concentrations:
10 g g–1 soil ,50 g g–1 soil ,100 g g–1 soil.
Reference substance (positive control):
no
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of nitrification rate
Remarks on result:
other: Potassium ethyl xanthate is a nitrification inhibitor that has a been observed to inhibit soil denitrification at concentrations around 50 ppm, but only in soils previously amended with mannitol to stimulate denitrifying activity
Details on results:
Potassium ethyl xanthate retarded denitrification when applied at the rate of 50 g g–1 soil to soil that had been amended with mannitol to promote microbial activity.
No inhibition of denitrification was observed when this compound was applied at the rate of 10 g g–1 soil, and enhancement of denitrification was observed when it was applied at the rate of 50 or 100 g g–1 soil.
The S-allyl O-pentyl dithiocarbonate (CAS No. 2956-12-9), the subject of this dossier) is expected to exhibit very similar toxicity to Potassium ethyl xanthate (CAS No. 140-89-6), which is also xanthate compound. Comparable metabolism would occur.




The influence of 28 nitrification inhibitors on denitrification of nitrate in soil was studied by determining the effects of different amounts of each inhibitor on the amounts of nitrate lost and the amounts of nitrite, N2O and N2 produced when soil samples were incubated anaerobically after treatment with nitrate or with nitrate and mannitol.

The inhibitors used included nitrapyrin (N-Serve), etridiazole (Dwell), potassium azide, 2-amino-4-chloro-6-methylpyrimidine (AM), sulfathiazole (ST), 4-amino-1,2,4-triazole(ATC),2,4-diamino-6-trichloromethyl-s-triazine (CL-1580), potassium ethyl xanthate, guanylthiourea (ASU), 4-nitrobenzotrichloride, 4-mesylbenzotrichloride, sodium thiocarbonate (STC), phenylmercuric acetate (PMA), and dicyandiamide (DCD).Only one of the nitrification inhibitors studied (potassium azide) retarded denitrification when applied at the rate of 10 g g–1 soil, and only two (potassium azide and 2,4-diamino-6-trichloromethyl-s-triazine) inhibited denitrification when applied at the rate of 50 g g–1 soil. The other inhibitors either had no appreciable effect on denitrification, or enhanced denitrification, when applied at the rate of 10 or 50 g g–1 soil, enhancement being most marked with 3-mercapto-1,2,4-triazole. Seven of the inhibitors (potassium azide, sulfathiazole,potassium ethyl xanthate,sodium isopropyl xanthate, 4-nitrobenzotrichloride, sodium thiocarbonate, and phenylmercuric acetate) retarded denitrification when applied at the rate of 50 g g–1 soil to soil that had been amended with mannitol to promote microbial activity.Reports that nitrapyrin (N-Serve) and etridiazole (Dwell) inhibit denitrification when applied at rates as low as 0.5 g g–1 soil could not be confirmed. No inhibition of denitrification was observed when these compounds were applied at the rate of 10 g g–1 soil, and enhancement of denitrification was observed when they were applied at the rate of 50 or 100 g g–1 soil.

Validity criteria fulfilled:
yes
Conclusions:
Potassium ethyl xanthate is a nitrification inhibitor that has been observed to inhibit soil denitrification at EC50= 50 mg/l.
The S-allyl O-pentyl dithiocarbonate (CAS No. 2956-12-9), the subject of this dossier) is expected to exhibit very similar toxicity to Potassium ethyl xanthate (CAS No. 140-89-6), which is also xanthate compound. Comparable metabolism would occur.
Executive summary:

Potassium ethyl xanthate retarded denitrification when applied at the rate of 50 g g–1 soil to soil that had been amended with mannitol to promote microbial activity. No inhibition of denitrification was observed when this compound was applied at the rate of 10 g g–1 soil, and enhancement of denitrification was observed when it was applied at the rate of 50 or 100 g g–1 soil.

The S-allyl O-pentyl dithiocarbonate (CAS No.2956-12-9), the subject of this dossier) is expected to exhibit very similar toxicity to Potassium ethyl xanthate (CAS No. 140-89-6), which is also xanthate compound. Comparable metabolism would occur.

Description of key information

Potassium ethyl xanthate is a nitrification inhibitor that has been observed to inhibit soil denitrification at EC50= 50 mg/l.

The S-allyl O-pentyl dithiocarbonate (CAS No. 2956-12-9), the subject of this dossier) is expected to exhibit very similar toxicity to Potassium ethyl xanthate  (CAS No. 140-89-6), which is also xanthate compound. Comparable metabolism would occur.

Key value for chemical safety assessment

EC50 for microorganisms:
50 mg/L
EC10 or NOEC for microorganisms:
10 mg/L

Additional information