3,7,11-trimethyldodeca-1,6,10-trien-3-ol,mixed isomers

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro DNA damage and/or repair study

Remarks:

Type of genotoxicity: DNA damage and/or repair

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable publication which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Modifying effects of components of plant essence on the induction of sister-chromatid exchanges in cultured Chinese hamster ovary cells

Author:

Sasaki YS, et al.

Year:

1989

Bibliographic source:

Mutation Research, 226, 103-110

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 479 (Genetic Toxicology: In Vitro Sister Chromatid Exchange Assay in Mammalian Cells)

Principles of method if other than guideline:

In principle the method descibed in OECD Guideline 479 was used with some deviations. Test substance was only investigated in absence of an appropriate metabolic system.

GLP compliance:

not specified

Type of assay:

sister chromatid exchange assay in mammalian cells

Test material

Test material information

Constituent 1

Reference substance name:

Linalool

EC Number:

201-134-4

EC Name:

Linalool

Cas Number:

78-70-6

Molecular formula:

C10H18O

IUPAC Name:

3,7-dimethylocta-1,6-dien-3-ol

Details on test material:

- Name of test material (as cited in study report): Linalool

Method

Species / strain

Species / strain / cell type:

Chinese hamster Ovary (CHO)

Metabolic activation:

without

Test concentrations with justification for top dose:

0, 33.3, 100, 333, 1000 µM

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Untreated negative controls:

other: not specified if solvent or negative control

Negative solvent / vehicle controls:

other: not specified if solvent or negative control

True negative controls:

no

Positive controls:

yes

Positive control substance:

mitomycin C

Details on test system and experimental conditions:

METHOD OF APPLICATION: in medium

DURATION
- Preincubation period: none
- Exposure duration: 21 hours

SPINDLE INHIBITOR (cytogenetic assays): colchicine

NUMBER OF CELLS EVALUATED: 50 per culture

Results and discussion

Test results

Species / strain:

Chinese hamster Ovary (CHO)

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

other: unclear if solvent or negative control

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Remarks on result:

other: other: CHO K-1 cells

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Component of plant essence	MMC treatment (0.15µM)	Mean frequency of SCEs
		Concentration  (µM) of components
		0	33.3	100	333	1000
Linalool	+	81.7	83.4	81.7	80.2	77.1
	-	9.0	9.0	8.9	8.8	9.0