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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Qualifier:
according to
Guideline:
other: Refer below principle
Principles of method if other than guideline:
Acute fish toxicity data of test substance 2-Methyl-4-nitrobenzenamine for exposure period of 96 hrs. on the basis of mortality effects.
GLP compliance:
not specified
Specific details on test material used for the study:
- IUPAC name 2-methyl-4-nitroaniline
- Name of test material: 4-nitro-o-toluidine
- Molecular formula: C7H8N2O2
- Molecular weight: 152.152 g/mol
- Physical state: Solid
- SMILES: c1(cc(c(N)cc1)C)[N+](=O)[O-]
- InChI: 1S/C7H8N2O2/c1-5-4-6(9(10)11)2-3-7(5)8/h2-4H,8H2,1H3
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name:Fathead Minnow)
- LIFE STAGE:Juvenile(s)
- Length at study initiation (length definition, mean, range and SD):2.4 CM
- Weight at study initiation (mean and range, SD):0.28 G
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
29.6 (12.0- 43.0)mg/L CaCO3
Test temperature:
20.3 (19.5-22.0) deg.C
pH:
7.6 (6.0- 9.2)
Dissolved oxygen:
7.2 (2.8-9.4) mg/L
Salinity:
ALKALINITY: 31.0 (15 - 60) mg/L CaCO3
Conductivity:
66.8 uS
Nominal and measured concentrations:
No data
Details on test conditions:
No data
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
86.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
not specified
Conclusions:
In acute fish toxicity study conducted for 2-methyl-4-nitroaniline, the median ethal concentration (LC50) on the basis of mortality effect for Pimephales promelas (Fathead Minnow) was observed to be 86.7 mg/l for exposure period of 96 hrs.
Executive summary:

Acute fish toxicity was conducted for 2-methyl-4-nitroaniline for exposure period of 96 hrs. Test organism was used as Pimephales promelas (Fathead Minnow)in static freshwater with test temp. 20.3 (19.5-22.0) deg.C ,hardness 29.6 (12.0- 43.0)mg/L CaCO3 ,pH7.6 (6.0- 9.2) , ALKALINITY:31.0 (15 - 60) mg/L CaCO3 ,conductivity 66.8 uS and dissolved oxygen 7.2 (2.8-9.4) mg/L. The median ethal concentration (LC50) on the basis of mortality effect for Pimephales promelas (Fathead Minnow) was observed to be 86.7 mg/l for exposure period of 96 hrs. Based on the value, the chemical was considered to be toxic to fish and can be considered to be classified in chronic category 3 as per the CLP regulations.

Description of key information

Acute fish toxicity was conducted for 2-methyl-4-nitroaniline. The median ethal concentration (LC50) on the basis of mortality effect for Pimephales promelas (Fathead Minnow) was observed to be 86.7 mg/l for exposure period of 96 hrs.

Key value for chemical safety assessment

LC50 for freshwater fish:
86.7 mg/L

Additional information

Acute fish toxicity was conducted for 2-methyl-4-nitroaniline for exposure period of 96 hrs. Test organism was used as Pimephales promelas (Fathead Minnow)in static freshwater with test temp. 20.3 (19.5-22.0) deg.C ,hardness 29.6 (12.0- 43.0)mg/L CaCO3 ,pH7.6 (6.0- 9.2) , ALKALINITY:31.0 (15 - 60) mg/L CaCO3 ,conductivity 66.8 uS and dissolved oxygen 7.2 (2.8-9.4) mg/L. The median ethal concentration (LC50) on the basis of mortality effect for Pimephales promelas (Fathead Minnow) was observed to be 86.7 mg/l for exposure period of 96 hrs. Based on the value, the chemical was considered to be toxic to fish and can be considered to be classified in chronic category 3 as per the CLP regulation.